Structure of PDB 1iup Chain A

Receptor sequence
>1iupA (length=271) Species: 294 (Pseudomonas fluorescens) [Search protein sequence]
NLEIGKSILAAGVLTNYHDVGEGQPVILIHGSGPGVSAYANWRLTIPALS
KFYRVIAPDMVGFGFTDRPENYNYSKDSWVDHIIGIMDALEIEKAHIVGN
AFGGGLAIATALRYSERVDRMVLMGAAGTRFDVTEGLNAVWGYTPSIENM
RNLLDIFAYDRSLVTDELARLRYEASIQPGFQESFSSMFPEPRQRWIDAL
ASSDEDIKTLPNETLIIHGREDQVVPLSSSLRLGELIDRAQLHVFGRCGH
WTQIEQTDRFNRLVVEFFNEA
3D structure
PDB1iup Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products
ChainA
Resolution1.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S34 G35 G37 N102 A103 F104 G127 V142 R174 F187 D224 H252 W253
Catalytic site (residue number reindexed from 1) S32 G33 G35 N100 A101 F102 G125 V140 R172 F185 D222 H250 W251
Enzyme Commision number 3.7.1.9: 2-hydroxymuconate-6-semialdehyde hydrolase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ALQ A Y76 W81 M190 W198 Y74 W79 M188 W196 MOAD: Ki=2.9mM
PDBbind-CN: -logKd/Ki=2.54,Ki=2.9mM
BS02 ALQ A S34 A103 F104 L139 W143 V226 H252 S32 A101 F102 L137 W141 V224 H250 MOAD: Ki=2.9mM
PDBbind-CN: -logKd/Ki=2.54,Ki=2.9mM
BS03 ALQ A A42 N43 R174 W253 A40 N41 R172 W251 MOAD: Ki=2.9mM
PDBbind-CN: -logKd/Ki=2.54,Ki=2.9mM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0016787 hydrolase activity

View graph for
Molecular Function
External links
PDB RCSB:1iup, PDBe:1iup, PDBj:1iup
PDBsum1iup
PubMed12192074
UniProtP96965

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