Structure of PDB 1iid Chain A

Receptor sequence

>1iidA (length=422) Species: 4932 (Saccharomyces cerevisiae)

AMKDHKFWRTQPVKDFDEKVVEEGPIDKPKTPEDISDKPLPLLSSFEWCS
IDVDNKKQLEDVFVLLNENYVEDRDAGFRFNYTKEFFNWALKSPGWKKDW
HIGVRVKETQKLVAFISAIPVTLGVRGKQVPSVEINFLCVHKQLRSKRLT
PVLIKEITRRVNKCDIWHALYTAGIVLPAPVSTCRYTHRPLNWKKLYEVD
FTGLPDGHTEEDMIAENALPAKTKTAGLRKLKKEDIDQVFELFKRYQSRF
ELIQIFTKEEFEHNFIGEESLPLDKQVIFSYVVEQPDGKITDFFSFYSLP
FTILNNTKYKDLGIGYLYYYATDADFQFKDRFDPKATKALKTRLCELIYD
ACILAKNANMDVFNALTSQDNTLFLDDLKFGPGDGFLNFYLFNYRAKPIT
GGLNPDNSNDIKRRSNVGVVML

3D structure

• PDB: 1iid Structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoylCoA and peptide provide insights about substrate recognition and catalysis.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N169 F170 L171 T205 L455
• Catalytic site (residue number reindexed from 1): N136 F137 L138 T172 L422
• Enzyme Commision number: 2.3.1.97: glycylpeptide N-tetradecanoyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	Y103 F113 Y219 H221 F334 Y349 G416 D417 G418 M454 L455	Y70 F80 Y186 H188 F301 Y316 G383 D384 G385 M421 L422
BS02	NHM	A	H38 K39 F40 W41 Y103 E105 I168 N169 L171 V173 R178 S179 R181 T183 P184 I187 T191 V194 A202 Y204 T205 I208 L210	H5 K6 F7 W8 Y70 E72 I135 N136 L138 V140 R145 S146 R148 T150 P151 I154 T158 V161 A169 Y171 T172 I175 L177

Gene Ontology

Molecular Function

• GO:0004379 glycylpeptide N-tetradecanoyltransferase activity
• GO:0016746 acyltransferase activity

Biological Process

• GO:0006499 N-terminal protein myristoylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1iid, PDBe:1iid, PDBj:1iid
• PDBsum: 1iid
• PubMed: 11371195
• UniProt: P14743|NMT_YEAST Glycylpeptide N-tetradecanoyltransferase (Gene Name=NMT1)