Structure of PDB 1ii0 Chain A

Receptor sequence

>1ii0A (length=550) Species: 562 (Escherichia coli)

MQFLQNIPPYLFFTGKGGVGKTSISCATAIRLAEQGKRVLLVSTDPASNV
GQVFSQTIGNTIQAIASVPGLSALEIDPQAAAQQYRARIVDPIKGVLPDD
VVSSINEQLSGACTTEIAAFDEFTGLLTDASLLTRFDHIIFDTAPTGHTI
RLLQLPGAWSSFIDSNPEGASCLGPMAGLEKQREQYAYAVEALSDPKRTR
LVLVARLQKSTLQEVARTHLELAAIGLKNQYLVINGVLPKTEAANDTLAA
AIWEREQEALANLPADLAGLPTDTLFLQPVNMVGVSALSRLLSTQPRPDI
PSLSALVDDIARNEHGLIMLMGKGGVGKTTMAAAIAVRLADMGFDVHLTT
SDPALNNLQVSRIDPHEETERYRQHVLETKGKELDEAGKRLLEEDLRSPC
TEEIAVFQAFSRVIREAGKRFVVMDTAPTGHTLLLLDATTTPMMLLQDPE
RTKVLLVTLPETTPVLEAANLQADLERAGIHPWGWIINNSLSIADTRSPL
LRMRAQQELPQIESVKRQHASRVALVPVLASEPTGIDKLKQLAGHHHHHH

3D structure

• PDB: 1ii0 Conformational changes in four regions of the Escherichia coli ArsA ATPase link ATP hydrolysis to ion translocation.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G18 G20 K21 T22 S23 D45 T502
• Catalytic site (residue number reindexed from 1): G18 G20 K21 T22 S23 D45 T463
• Enzyme Commision number: 7.3.2.7: arsenite-transporting ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	T22 D45	T22 D45
BS02	ADP	A	G18 V19 G20 K21 T22 S23 N235 G236 L277 Q278 N281 M282 T502	G18 V19 G20 K21 T22 S23 N235 G236 L277 Q278 N281 M282 T463
BS03	ATP	A	G336 G337 G339 K340 T341 T342 N527 L568 P572	G324 G325 G327 K328 T329 T330 N488 L529 P533
BS04	TAS	A	R206 E500	R206 E461

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0015446 ATPase-coupled arsenite transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0046685 response to arsenic-containing substance
• GO:0071722 detoxification of arsenic-containing substance

External links

• PDB: RCSB:1ii0, PDBe:1ii0, PDBj:1ii0
• PDBsum: 1ii0
• PubMed: 11395509
• UniProt: P08690|ARSA1_ECOLX Arsenical pump-driving ATPase (Gene Name=arsA)