Structure of PDB 1ih8 Chain A

Receptor sequence
>1ih8A (length=271) Species: 1423 (Bacillus subtilis) [Search protein sequence]
SMQEKIMRELHVKPSIDPKQEIEDRVNFLKQYVKKTGAKGFVLGISGGQD
STLAGRLAQLAVESIREEGGDAQFIAVRLPHGTQQDEDDAQLALKFIKPD
KSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNVKARTRMIAQYAIGGQE
GLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLKELGAPE
RLYLKEPTADLLDEKPQQSDETELGISYDEIDDYLEGKEVSAKVSEALEK
RYSMTEHKRQVPASMFDDWWK
3D structure
PDB1ih8 Stabilization of active-site loops in NH3-dependent NAD+ synthetase from Bacillus subtilis.
ChainA
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D50 E162
Catalytic site (residue number reindexed from 1) D50 E162
Enzyme Commision number 6.3.1.5: NAD(+) synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 APC A G44 I45 S46 G48 Q49 D50 S51 R78 L79 Q84 R139 P207 T208 G44 I45 S46 G48 Q49 D50 S51 R78 L79 Q84 R139 P207 T208
Gene Ontology
Molecular Function
GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
GO:0004359 glutaminase activity
GO:0005524 ATP binding
GO:0008795 NAD+ synthase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0009435 NAD biosynthetic process
GO:0030435 sporulation resulting in formation of a cellular spore
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1ih8, PDBe:1ih8, PDBj:1ih8
PDBsum1ih8
PubMed11375500
UniProtP08164|NADE_BACSU NH(3)-dependent NAD(+) synthetase (Gene Name=nadE)

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