Structure of PDB 1hzv Chain A

Receptor sequence

>1hzvA (length=514) Species: 287 (Pseudomonas aeruginosa)

VRTNGAPDMSESEFNEAKQIYFQRCAGCHGVLRKGATGKPLTPDITQQRG
QQYLEALITYGTPLGMELSKEQITLMAKYIQHTPPQPPEWGMPEMRESWK
VLVKPEDRPKKQLNDLDLPNLFSVTLRDAGQIALVDGDSKKIVKVIDTGY
AVHISRMSASGRYLLVIGRDARIDMIDLWAKEPTKVAEIKIGIEARSVES
SKFKGYEDRYTIAGAYWPPQFAIMDGETLEPKQIVSTRGMTVDTQTYHPE
PRVAAIIASHEHPEFIVNVKETGKVLLVNYKDIDNLTVTSIGAAPFLHDG
GWDSSHRYFMTAANNSNKVAVIDSKDRRLSALVDVGKTPAPGRGANFVHP
KYGPVWSTSHLGDGSISLIGTDPKNHPQYAWKKVAELQGQGGGSLFIKTH
PKSSHLYVDTTFNPDARISQSVAVFDLKNLDAKYQVLPIAEWADLGEGAK
RVVQPEYNKRGDEVWFSVWNGKNDSSALVVVDDKTLKLKAVVKDPRLITP
TGKFNVYNTQHDVY

3D structure

• PDB: 1hzv Domain swing upon His to Ala mutation in nitrite reductase of Pseudomonas aeruginosa.
• Chain: A
• Resolution: 2.83 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C47 C50 H51 M88 H327 A369
• Catalytic site (residue number reindexed from 1): C25 C28 H29 M66 H298 A340
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	R46 C47 C50 H51 R71 L79 T84 L86 G87 M105	R24 C25 C28 H29 R49 L57 T62 L64 G65 M76
BS02	DHE	A	R156 H182 I183 R185 R198 R225 S226 Y245 A283 A284 I285 H327 R372 F425 Q483 W498 F533	R127 H153 I154 R156 R169 R196 S197 Y216 A254 A255 I256 H298 R343 F396 Q454 W469 F504

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:1hzv, PDBe:1hzv, PDBj:1hzv
• PDBsum: 1hzv
• PubMed: 11563915
• UniProt: P24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)