Structure of PDB 1hzu Chain A

Receptor sequence

>1hzuA (length=521) Species: 287 (Pseudomonas aeruginosa)

VRTNGAPDMSESEFNEAKQIYFQRCAGCHGVLRKGATGKPLTPDITQQRG
QQYLEALITYGTPLGMPNWGSSGELSKEQITLMAKYIQHTPPQPPEWGMP
EMRESWKVLVKPEDRPKKQLNDLDLPNLFSVTLRDAGQIALVDGDSKKIV
KVIDTGYAVHISRMSASGRYLLVIGRDARIDMIDLWAKEPTKVAEIKIGI
EARSVESSKFKGYEDRYTIAGAYWPPQFAIMDGETLEPKQIVSTRGMTVD
TQTYHPEPRVAAIIASHEHPEFIVNVKETGKVLLVNYKDIDNLTVTSIGA
APFLADGGWDSSHRYFMTAANNSNKVAVIDSKDRRLSALVDVGKTPHPGR
GANFVHPKYGPVWSTSHLGDGSISLIGTDPKNHPQYAWKKVAELQGQGGG
SLFIKTHPKSSHLYVDTTFNPDARISQSVAVFDLKNLDAKYQVLPIAEWA
DLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDSSALVVVDDKTLKLKAVVK
DPRLITPTGKFNVYNTQHDVY

3D structure

• PDB: 1hzu Domain swing upon His to Ala mutation in nitrite reductase of Pseudomonas aeruginosa.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C47 C50 H51 M88 A327 H369
• Catalytic site (residue number reindexed from 1): C25 C28 H29 M66 A305 H347
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	C47 C50 H51 G60 K61 L63 R71 L79 T84 L86 M88 P89	C25 C28 H29 G38 K39 L41 R49 L57 T62 L64 M66 P67
BS02	DHE	A	R156 H182 I183 R185 R225 I285 D328 R372 F425 T530 F533	R134 H160 I161 R163 R203 I263 D306 R350 F403 T508 F511

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:1hzu, PDBe:1hzu, PDBj:1hzu
• PDBsum: 1hzu
• PubMed: 11563915
• UniProt: P24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)