Structure of PDB 1hyu Chain A

Receptor sequence

>1hyuA (length=521)

MLDTNMKTQLRAYLEKLTKPVELIATLDDSAKSAEIKELLAEIAELSDKV
TFKEDNTLPVRKPSFLITNPGSQQGPRFAGSPLGHEFTSLVLALLWTGGH
PSKEAQSLLEQIRDIDGDFEFETYYSLSCHNCPDVVQALNLMAVLNPRIK
HTAIDGGTFQNEITERNVMGVPAVFVNGKEFGQGRMTLTEIVAKVDTGAE
KRAAEALNKRDAYDVLIVGSGPAGAAAAVYSARKGIRTGLMGERFGGQVL
DTVDIENYISVPKTEGQKLAGALKAHVSDYDVDVIDSQSASKLVPAATEG
GLHQIETASGAVLKARSIIIATGAKWRNMNVPGEDQYRTKGVTYCPHCDG
PLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKADQVLQDKV
RSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHSVALAGIFVQIG
LLPNTHWLEGALERNRMGEIIIDAKCETSVKGVFAAGDCTTVPYKQIIIA
TGEGAKASLSAFDYLIRTKIA

3D structure

• PDB: 1hyu Structure of intact AhpF reveals a mirrored thioredoxin-like active site and implies large domain rotations during catalysis.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 1.8.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	G221 P222 A223 E243 G247 Q248 N257 S289 A290 T322 G323 W326 C345 D488 K495 Q496 I497	G221 P222 A223 E243 G247 Q248 N257 S289 A290 T322 G323 W326 C345 D488 K495 Q496 I497

Gene Ontology

Molecular Function

• GO:0004791 thioredoxin-disulfide reductase (NADPH) activity
• GO:0008785 alkyl hydroperoxide reductase activity
• GO:0016491 oxidoreductase activity
• GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
• GO:0050660 flavin adenine dinucleotide binding
• GO:0051287 NAD binding

Biological Process

• GO:0000302 response to reactive oxygen species
• GO:0045454 cell redox homeostasis
• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1hyu, PDBe:1hyu, PDBj:1hyu
• PDBsum: 1hyu
• PubMed: 11300769
• UniProt: P19480|AHPF_SALTY Alkyl hydroperoxide reductase subunit F (Gene Name=ahpF)