Structure of PDB 1hyg Chain A

Receptor sequence

>1hygA (length=313) Species: 2190 (Methanocaldococcus jannaschii)

MKVTIIGASGRVGSATALLLAKEPFMKDLVLIGREHSINKLEGLREDIYD
ALAGTRSDANIYVESDENLRIIDESDVVIITSGVPRKEGMSRMDLAKTNA
KIVGKYAKKIAEICDTKIFVITNPVDVMTYKALVDSKFERNQVFGLGTHL
DSLRFKVAIAKFFGVHIDEVRTRIIGEHGDSMVPLLSATSIGGIPIQKFE
RFKELPIDEIIEDVKTKGEQIIRLKGGSEFGPAAAILNVVRCIVNNEKRL
LTLSAYVDGEFDGIRDVCIGVPVKIGRDGIEEVVSIELDKDEIIAFRKSA
EIIKKYCEEVKNL

3D structure

• PDB: 1hyg Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R92 D151 R154 H178
• Catalytic site (residue number reindexed from 1): R92 D151 R154 H178
• Enzyme Commision number: 1.1.1.375: L-2-hydroxycarboxylate dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAP	A	G7 S9 G10 R11 R34 S37 T81 G83 T122 N123 G227 S228	G7 S9 G10 R11 R34 S37 T81 G83 T122 N123 G227 S228

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0102443 L-2-hydroxycarboxylate dehydrogenase (NAD+) activity

Biological Process

• GO:0006089 lactate metabolic process
• GO:0006090 pyruvate metabolic process
• GO:0006099 tricarboxylic acid cycle
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1hyg, PDBe:1hyg, PDBj:1hyg
• PDBsum: 1hyg
• PubMed: 11292347
• UniProt: Q60176|MDH_METJA L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+)) (Gene Name=mdh)