Structure of PDB 1hx9 Chain A

Receptor sequence
>1hx9A (length=523) Species: 4097 (Nicotiana tabacum) [Search protein sequence]
SPSLWGDQFLSFSIDNQVAEKYAKEIEALKEQTRNMLLATGMKLADTLNL
IDTIERLGISYHFEKEIDDILDQIYNQDLCTSALQFRLLRQHGFNISPEI
FSKFQDENGKFKESLASDVLGLLNLYEASHVRTHADDILEDALAFSTIHL
ESAAPHLKSPLREQVTHALEQCLHKGVPRVETRFFISSIYDKEQSKNNVL
LRFAKLDFNLLQMLHKQELAQVSRWWKDLDFVTTLPYARDRVVECYFSAL
GVYFEPQYSQARVMLVKTISMISIVDDTFDAYGTVKELEAYTDAIQRWDI
NEIDRLPDYMKISYKAILDLYKDYEKELSSAGRSHIVCHAIERMKEVVRN
YNVESTWFIEGYTPPVSEYLSNALATTTYYYLATTSYLGMKSATEQDFEW
LSKNPKILEASVIICRVIDDTATYEVEKSRGQIATGIECCMRDYGISTKE
AMAKFQNMAETAWKDINEGLLRPTPVSTEFLTPILNLARIVEVTYIHNLD
GYTHPEEVLKPHIINLLVDSIKI
3D structure
PDB1hx9 Single Active Site Mutations Change the Specificity of a Sesquiterpene Cyclase
ChainA
Resolution3.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R264 S273 I294 D301 D305 T401 T402 T403 R441 D444 T448 E452 Y520 D525 Y527
Catalytic site (residue number reindexed from 1) R239 S248 I269 D276 D280 T376 T377 T378 R416 D419 T423 E427 Y495 D500 Y502
Enzyme Commision number 4.2.3.61: 5-epiaristolochene synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D444 E452 D419 E427
BS02 MG A D301 D305 E379 D276 D280 E354
BS03 FHP A R264 D301 Y404 Y520 Y527 R239 D276 Y379 Y495 Y502
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0010333 terpene synthase activity
GO:0016829 lyase activity
GO:0016838 carbon-oxygen lyase activity, acting on phosphates
GO:0046872 metal ion binding
GO:0102698 5-epi-aristolochene synthase activity
Biological Process
GO:0006720 isoprenoid metabolic process
GO:0016102 diterpenoid biosynthetic process
GO:0016114 terpenoid biosynthetic process
GO:0051762 sesquiterpene biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:1hx9, PDBe:1hx9, PDBj:1hx9
PDBsum1hx9
PubMed
UniProtQ40577|5EAS_TOBAC 5-epi-aristolochene synthase (Gene Name=EAS3)

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