Structure of PDB 1hwl Chain A

Receptor sequence

>1hwlA (length=407) Species: 9606 (Homo sapiens)

PRPNEECFLSDAEIIQLVNAKHIPAYKLETLIETHERGVSIRRQLLSKKL
SEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQV
PMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSA
EVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGD
AMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRG
KSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHA
ANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIG
TVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSL
MAALAAG

3D structure

• PDB: 1hwl Structural mechanism for statin inhibition of HMG-CoA reductase.
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E559 K691 D767
• Catalytic site (residue number reindexed from 1): E106 K238 D314
• Enzyme Commision number: 1.1.1.34: hydroxymethylglutaryl-CoA reductase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	Y479 N529	Y26 N76
BS02	ADP	A	A564 N567 R568 K722	A111 N114 R115 K269
BS03	FBI	A	R590 S684 D690 K691 K692	R137 S231 D237 K238 K239	MOAD: ic50=5nM
BS04	FBI	A	E559 C561 L562 S565 K735 A751 L853 A856	E106 C108 L109 S112 K282 A298 L400 A403	MOAD: ic50=5nM

Gene Ontology

Molecular Function

• GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0050661 NADP binding

Biological Process

• GO:0008299 isoprenoid biosynthetic process
• GO:0015936 coenzyme A metabolic process

Cellular Component

• GO:0005789 endoplasmic reticulum membrane

External links

• PDB: RCSB:1hwl, PDBe:1hwl, PDBj:1hwl
• PDBsum: 1hwl
• PubMed: 11349148
• UniProt: P04035|HMDH_HUMAN 3-hydroxy-3-methylglutaryl-coenzyme A reductase (Gene Name=HMGCR)