Structure of PDB 1hmu Chain A

Receptor sequence

>1hmuA (length=674) Species: 984 (Pedobacter heparinus)

GTAELIMKRVMLDLKKPLRNMDKVAEKNLNTLQPDGSWKDVPYKDDAMTN
WLPNNHLLQLETIIQAYIEKDSHYYGDDKVFDQISKAFKYWYDSDPKSRN
WWHNEIATPQALGEMLILMRYGKKPLDEALVHKLTERMKRGEPEKKTGAN
KTDIALHYFYRALLTSDEALLSFAVKELFYPVQFVHYEEGLQYDYSYLQH
GPQLQISSYGAVFITGVLKLANYVRDTPYALSTEKLAIFSKYYRDSYLKA
IRGSYMDFNVEGRGVSRPDILNKKAEKKRLLVAKMIDLKHTEEWADAIAR
TDSTVAAGYKIEPYHHQFWNGDYVQHLRPAYSFNVRMVSKRTRRSESGNK
ENLLGRYLSDGATNIQLRGPEYYNIMPVWEWDKIPGITSRDYLTDRPLTK
LWGEQGSNDFAGGVSDGVYGASAYALDYDSLQAKKAWFFFDKEIVCLGAG
INSNAPENITTTLNQSWLNGPVISTAGKTGRGKITTFKAQGQFWLLHDAI
GYYFPEGANLSLSTQSQKGNWFHINNSHSKDEVSGDVFKLWINHGARPEN
AQYAYIVLPGINKPEEIKKYNGTAPKVLANTNQLQAVYHQQLDMVQAIFY
TAGKLSVAGIEIETDKPCAVLIKHINGKQVIWAADPLQKEKTAVLSIRDL
KTGKTNRVKIDFPQQEFAGATVEL

3D structure

• PDB: 1hmu Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N175 H225 Y234 R288 E371
• Catalytic site (residue number reindexed from 1): N150 H200 Y209 R263 E346
• Enzyme Commision number: 4.2.2.5: chondroitin AC lyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	A	S455 N479	S430 N454
BS02	GCU	A	R415 R421	R390 R396
BS03	ASG	A	W127 S233 Y234 R288 R292	W102 S208 Y209 R263 R267
BS04	CA	A	E405 D407 D416 Y417	E380 D382 D391 Y392

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016829 lyase activity
• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:1hmu, PDBe:1hmu, PDBj:1hmu
• PDBsum: 1hmu
• PubMed: 11327856
• UniProt: Q59288|CSLA_PEDHD Chondroitinase-AC (Gene Name=cslA)