Structure of PDB 1h9y Chain A

Receptor sequence

>1h9yA (length=520) Species: 82367 (Paracoccus pantotrophus)

ALSDAQYNEANKIYFERCAGCHGVLRKGATGKALTPDLTRDLGFDYLQSF
ITYGSPAGMPNWGTSGELSAEQVDLMANYLLLDPAAPPEFGMKEMRESWK
VHVAPEDRPTQQENDWDLENLFSVTLRDAGQIALIDGATYEIKSVLDTGY
AVHISRLSASGRYLFVIGRDGKVNMIDLWMKEPTTVAEIKIGSEARSIET
SKMEGWEDKYAIAGAYWPPQYVIMDGETLEPKKIQSTRGMTYDEQEYHPE
PRVAAILASHYRPEFIVNVKETGKILLVDYTDLDNLKTTEISAERFLHDG
GLDGSHRYFITAANARNKLVVIDTKEGKLVAIEDTGGQTPHPGRGANFVH
PTFGPVWATSHMGDDSVALIGTDPEGHPDNAWKILDSFPALGGGSLFIKT
HPNSQYLYVDATLNPEAEISGSVAVFDIKAMTGDGSDPEFKTLPIAEWAG
ITEGQPRVVQGEFNKDGTEVWFSVWNGKDQESALVVVDDKTLELKHVIKD
ERLVTPTGKFNVYNTMTDTY

3D structure

• PDB: 1h9y The Structure of an Alternative Form of Paracoccus Pantotrophus Cytochrome Cd1 Nitrite Reductase
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C65 C68 H69 M106 H345 H388
• Catalytic site (residue number reindexed from 1): C18 C21 H22 M59 H298 H341
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	C65 C68 H69 G78 K79 L81 Y93 F97 I98 S102 A104 G105 M106 P107 W109	C18 C21 H22 G31 K32 L34 Y46 F50 I51 S55 A57 G58 M59 P60 W62
BS02	DHE	A	R174 H200 I201 R216 R243 S244 I245 Y263 A301 A302 H345 R391 F444 T554 F557	R127 H153 I154 R169 R196 S197 I198 Y216 A254 A255 H298 R344 F397 T507 F510

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:1h9y, PDBe:1h9y, PDBj:1h9y
• PDBsum: 1h9y
• PubMed: 11373294
• UniProt: P72181|NIRS_PARPN Nitrite reductase (Gene Name=nirS)