Structure of PDB 1gz3 Chain A

Receptor sequence
>1gz3A (length=554) Species: 9606 (Homo sapiens) [Search protein sequence]
HIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFH
RNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLA
CSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGL
GDLGVYGMGIPVGKLCLYTACAGIRPDQCLPVCIDVGTDNIALLKDPFYM
GLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKY
REKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGI
ANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSA
PESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFAL
SNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVY
IFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANI
QEVSINIAIKVTEYLYANKMAFVYPEPEDKAKYVKEQTWRSEYDSLLPDV
YEWP
3D structure
PDB1gz3 Molecular Mechanism for the Regulation of Human Mitochondrial Nad(P)+-Dependent Malic Enzyme by ATP and Fumarate
ChainA
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y112 R165 K183 E255 D256 D278 D279 N421
Catalytic site (residue number reindexed from 1) Y93 R146 K164 E236 D237 D259 D260 N402
Enzyme Commision number 1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A R165 N259 A312 G313 E314 A315 D345 K346 V392 A393 G394 L398 R146 N240 A293 G294 E295 A296 D326 K327 V373 A374 G375 L379 MOAD: Ki=0.2mM
PDBbind-CN: -logKd/Ki=3.70,Ki=0.20mM
BS02 OXL A R165 L167 K183 N421 N467 R146 L148 K164 N402 N448
BS03 MN A E255 D256 D279 E236 D237 D260
BS04 FUM A R67 R91 R48 R72
Gene Ontology
Molecular Function
GO:0004470 malic enzyme activity
GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948 oxaloacetate decarboxylase activity
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872 metal ion binding
GO:0051287 NAD binding
Biological Process
GO:0006090 pyruvate metabolic process
GO:0006108 malate metabolic process
GO:1902031 regulation of NADP metabolic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1gz3, PDBe:1gz3, PDBj:1gz3
PDBsum1gz3
PubMed12121650
UniProtP23368|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)

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