Structure of PDB 1gwh Chain A

Receptor sequence

>1gwhA (length=498) Species: 1270 (Micrococcus luteus)

TTPHATGSTRQNGAPAVSDRQSLTVGSEGPIVLHDTHLLETHQHFNRMNI
PERRPHAKGSGAFGEFEVTEDVSKYTKALVFQPGTKTETLLRFSTVAGEL
GSPDTWRDVRGFALRFYTEEGNYDLVGNNTPIFFLRDPMKFTHFIRSQKR
LPDSGLRDATMQWDFWTNNPESAHQVTYLMGPRGLPRTWREMNGYGSHTY
LWVNAQGEKHWVKYHFISQQGVHNLSNDEATKIAGENADFHRQDLFESIA
KGDHPKWDLYIQAIPYEEGKTYRFNPFDLTKTISQKDYPRIKVGTLTLNR
NPENHFAQIESAAFSPSNTVPGIGLSPDRMLLGRAFAYHDAQLYRVGAHV
NQLPVNRPKNAVHNYAFEGQMWYDHTGDRSTYVPNSNGDSWSDETGPVDD
GWEADGTLTREAQALRADDDDFGQAGTLVREVFSDQERDDFVETVAGALK
GVRQDVQARAFEYWKNVDATIGQRIEDEVKRHEGDGIPGVEAGGEARI

3D structure

• PDB: 1gwh The Structures of Micrococcus Lysodeikticus Catalase, its Ferryl Intermediate (Compound II) and Nadph Complex.
• Chain: A
• Resolution: 1.74 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H61 N133 S320
• Catalytic site (residue number reindexed from 1): H56 N128 S315
• Enzyme Commision number: 1.11.1.6: catalase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	R58 R59 P60 H61 R97 V131 G132 N133 P143 F146 S202 F319 M335 R339 Y343 Q347 R350	R53 R54 P55 H56 R92 V126 G127 N128 P138 F141 S197 F314 M330 R334 Y338 Q342 R345
BS02	NDP	A	Y183 R188 Y200 Q290 K291 R295 L433 V437	Y178 R183 Y195 Q285 K286 R290 L428 V432

Gene Ontology

Molecular Function

• GO:0004096 catalase activity
• GO:0004601 peroxidase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006979 response to oxidative stress
• GO:0042542 response to hydrogen peroxide
• GO:0042744 hydrogen peroxide catabolic process
• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1gwh, PDBe:1gwh, PDBj:1gwh
• PDBsum: 1gwh
• PubMed: 12454454
• UniProt: P29422|CATA_MICLU Catalase (Gene Name=katA)