Structure of PDB 1gw1 Chain A

Receptor sequence

>1gw1A (length=375) Species: 155077 (Cellvibrio japonicus)

KPVTVKLVDSQATMETRSLFAFMQEQRRHSIMFGHQHETTQGLTITRTDG
TQSDTFNAVGDFAAVYGWDTLSIVAPKAEGDIVAQVKKAYARGGIITVSS
HFDNPKTDTQKGVWPVGTSWDQTPAVVDSLPGGAYNPVLNGYLDQVAEWA
NNLKDEQGRLIPVIFRLYHANTGSWFWWGDKQSTPEQYKQLFRYSVEYLR
DVKGVRNFLYAYSPNNFWDVTEANYLERYPGDEWVDVLGFDTYGPVADNA
DWFRNVVANAALVARMAEARGKIPVISEIGIRAPDIEAGLYDNQWYRKLI
SGLKADPDAREIAFLLVWRNAPQGVPGQVPHYWVPANRPENINNGTLEDF
QAFYADEFTAFNRDIEQVYQRPTLI

3D structure

• PDB: 1gw1 Substrate Distortion by a Beta-Mannanase: Snapshots of the Michaelis and Covalent-Intermediate Complexes Suggest a B2,5 Conformation for the Transition State
• Chain: A
• Resolution: 1.65 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.78: mannan endo-1,4-beta-mannosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAF	A	H143 H211 Y285 E320 W360	H101 H169 Y243 E278 W318
BS02	BMA	A	E121 W162 W360 R361 H377	E79 W120 W318 R319 H331
BS03	ZN	A	E67 H71	E25 H29
BS04	ZN	A	R208 H211 D283 E320	R166 H169 D241 E278

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016787 hydrolase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0016985 mannan endo-1,4-beta-mannosidase activity

Biological Process

• GO:0000272 polysaccharide catabolic process
• GO:0006080 substituted mannan metabolic process
• GO:0010391 glucomannan metabolic process
• GO:0051069 galactomannan metabolic process

External links

• PDB: RCSB:1gw1, PDBe:1gw1, PDBj:1gw1
• PDBsum: 1gw1
• PubMed: 12203498
• UniProt: P49424|MANA_CELJU Mannan endo-1,4-beta-mannosidase (Gene Name=manA)