Structure of PDB 1gra Chain A

Receptor sequence

>1graA (length=461) Species: 9606 (Homo sapiens) [Search protein sequence]

VASYDYLVIGGGSGGLASARRAAELGARAAVVESHKLGGTCVNVGCVPKK
VMWNTAVHSEFMHDHADYGFPSCEGKFNWRVIKEKRDAYVSRLNAIYQNN
LTKSHIEIIRGHAAFTSDPKPTIEVSGKKYTAPHILIATGGMPSTPHESQ
IPGASLGITSDGFFQLEELPGRSVIVGAGYIAVEMAGILSALGSKTSLMI
RHDKVLRSFDSMISTNCTEELENAGVEVLKFSQVKEVKKTLSGLEVSMVT
AVPGRLPVMTMIPDVDCLLWAIGRVPNTKDLSLNKLGIQTDDKGHIIVDE
FQNTNVKGIYAVGDVCGKALLTPVAIAAGRKLAHRLFEYKEDSKLDYNNI
PTVVFSHPPIGTVGLTEDEAIHKYGIENVKTYSTSFTPMYHAVTKRKTKC
VMKMVCANKEEKVVGIHMQGLGCDEMLQGFAVAVKMGATKADFDNTVAIH
PTSSEELVTLR

3D structure

PDB

1gra Substrate binding and catalysis by glutathione reductase as derived from refined enzyme: substrate crystal structures at 2 A resolution.

Chain

Resolution

2.0 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	L54 C58 C63 K66 Y197 E201 A465 H467 E472
Catalytic site (residue number reindexed from 1)	L37 C41 C46 K49 Y180 E184 A448 H450 E455
Enzyme Commision number	1.8.1.7: glutathione-disulfide reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	FAD	A	G29 S30 G31 E50 S51 G56 T57 C58 G62 C63 K66 H129 A130 T156 G157 Y197 R291 G330 D331 L337 L338 T339	G12 S13 G14 E33 S34 G39 T40 C41 G45 C46 K49 H112 A113 T139 G140 Y180 R274 G313 D314 L320 L321 T322
BS02	NDP	A	A195 G196 R218 R224 I289	A178 G179 R201 R207 I272
BS03	GSH	A	R37 V59 Y114 I343 R347	R20 V42 Y97 I326 R330
BS04	GSH	A	I113 Y114	I96 Y97

Gene Ontology

	Molecular Function
GO:0004362	glutathione-disulfide reductase (NADPH) activity
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0016668	oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding

	Biological Process
GO:0006749	glutathione metabolic process
GO:0034599	cellular response to oxidative stress
GO:0045454	cell redox homeostasis
GO:0098869	cellular oxidant detoxification

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005759	mitochondrial matrix
GO:0005829	cytosol
GO:0009897	external side of plasma membrane
GO:0070062	extracellular exosome

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1gra, PDBe:1gra, PDBj:1gra
PDBsum	1gra
PubMed	2585516
UniProt	P00390\|GSHR_HUMAN Glutathione reductase, mitochondrial (Gene Name=GSR)

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