Structure of PDB 1gar Chain A

Receptor sequence

>1garA (length=204) Species: 562 (Escherichia coli)

MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLERARQAG
IATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHY
AGRLLNIHPSLLPKYPGLHTHNGDEEHGTSVHFVTDELDGGPVILQAKVP
VFAGDSEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPP
QGYA

3D structure

• PDB: 1gar Towards structure-based drug design: crystal structure of a multisubstrate adduct complex of glycinamide ribonucleotide transformylase at 1.96 A resolution.
• Chain: A
• Resolution: 1.96 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N106 H108 S135 D144
• Catalytic site (residue number reindexed from 1): N106 H108 S130 D139
• Enzyme Commision number: 2.1.2.2: phosphoribosylglycinamide formyltransferase 1.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	U89	A	N10 G11 S12 N13 R64 G87 M89 R90 I91 L92 N106 I107 H108 P109 L143 D144 E173	N10 G11 S12 N13 R64 G87 M89 R90 I91 L92 N106 I107 H108 P109 L138 D139 E168	MOAD: Ki=100pM PDBbind-CN: -logKd/Ki=10.00,Ki=100pM

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004644 phosphoribosylglycinamide formyltransferase activity
• GO:0016740 transferase activity

Biological Process

• GO:0006164 purine nucleotide biosynthetic process
• GO:0006189 'de novo' IMP biosynthetic process
• GO:0006974 DNA damage response
• GO:0009058 biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1gar, PDBe:1gar, PDBj:1gar
• PDBsum: 1gar
• PubMed: 7776369
• UniProt: P08179|PUR3_ECOLI Phosphoribosylglycinamide formyltransferase (Gene Name=purN)