Structure of PDB 1g8j Chain A

Receptor sequence

>1g8jA (length=820) Species: 511 (Alcaligenes faecalis)

RITLPPANAQRTNMTCHFCIVGCGYHVYKWPELEEGGRAPEQNALGLDFR
KQLPPLASTLTPAMTNVVTEHDGARYDIMVVPDKACVVNSGLSSTRGGKM
ASYMYTPTGDGKERLSAPRLYAADEWVDTTWDHAMALYAGLIKKTLDSDG
PQGVFFSCFDHGGAGGGFENTWGTGKLMFSAIQTPMVRIHNRPAYNSECH
ATREMGIGELNNAYEDAQLADVIWSIGNNPYESQTNYFLNHWLPNLQGAT
TSKKKERFPNENFPQARIIFVDPRETPSVAIARHVAGNDRVLHLAIEPGT
DTALFNGLFTYVVEQGWIDKPFIEAHTKGFDDAVKTNRLSLDECSNITGV
PVDMLKRAAEWSYKPKASGQAPRTMHAYEKGIIWGNDNYVIQSALLDLVI
ATHNVGRRGTGCVRMGGHQEGYTRPPYPGDKKIYIDQELIKGKGRIMTWW
GCNNFQTSNNAQALREAILQRSAIVKQAMQKARGATTEEMVDVIYEATQN
GGLFVTSINLYPTKLAEAAHLMLPAAHPGEMNLTSMNGERRIRLSEKFMD
PPGTAMADCLIAARIANALRDMYQKDGKAEMAAQFEGFDWKTEEDAFNDG
FRRAGQPGAPAIDSQGGSTGHLVTYDRLRKSGNNGVQLPVVSWDESKGLV
GTEMLYTEGKFDTDDGKAHFKPAPWNGLPATVQQQKDKYRFWLNNGRNNE
VWQTAYHDQYNSLMQERYPMAYIEMNPDDCKQLDVTGGDIVEVYNDFGST
FAMVYPVAEIKRGQTFMLFGYVNGIQGDVTTDWTDRDIIPYYKGTWGDIR
KVGSMSEFKRTVSFKSRRFG

3D structure

• PDB: 1g8j Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A.
• Chain: A
• Resolution: 2.03 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C24 S98 S99 R101 A199 Y200 S238 G421
• Catalytic site (residue number reindexed from 1): C19 S93 S94 R96 A194 Y195 S233 G416
• Enzyme Commision number: 1.20.9.1: arsenate reductase (azurin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MGD	A	G170 N196 K385 W389 G456 C457 N458 T462 I513 N514 L515 A530 D563 N700 R702 Q708 T709 Y711 F774 Y797 K798	G165 N191 K380 W384 G451 C452 N453 T457 I508 N509 L510 A525 D558 N695 R697 Q703 T704 Y706 F769 Y792 K793
BS02	MGD	A	R101 G232 N233 E237 S238 D277 P278 R279 G304 D306 E384 K385 G386 G422 W697 N699 G701 R702 N704 V706 W707 Q708 K798	R96 G227 N228 E232 S233 D272 P273 R274 G299 D301 E379 K380 G381 G417 W692 N694 G696 R697 N699 V701 W702 Q703 K793
BS03	F3S	A	C21 C24 V26 G27 C28 S99 R101 G102 N241	C16 C19 V21 G22 C23 S94 R96 G97 N236

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0050611 arsenate reductase (azurin) activity
• GO:0051536 iron-sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding

Biological Process

• GO:0045333 cellular respiration

Cellular Component

• GO:1990204 oxidoreductase complex

External links

• PDB: RCSB:1g8j, PDBe:1g8j, PDBj:1g8j
• PDBsum: 1g8j
• PubMed: 11250197
• UniProt: Q7SIF4|AIOA_ALCFA Arsenite oxidase subunit AioA (Gene Name=aioA)