Structure of PDB 1g59 Chain A

Receptor sequence

>1g59A (length=468) Species: 274 (Thermus thermophilus) [Search protein sequence]

MVVTRIAPSPTGDPHVGTAYIALFNYAWARRNGGRFIVRIEDTDRARYVP
GAEERILAALKWLGLSYDEGPDVAAPTGPYRQSERLPLYQKYAEELLKRG
WAYRAFETPEELEQIRKEKGGYDGRARNIPPEEAEERARRGEPHVIRLKV
PRPGTTEVKDELRGVVVYDNQEIPDVVLLKSDGYPTYHLANVVDDHLMGV
TDVIRAEEWLVSTPIHVLLYRAFGWEAPRFYHMPLLRNPDKTKISKRKSH
TSLDWYKAEGFLPEALRNYLCLMGFSMPDGREIFTLEEFIQAFTWERVSL
GGPVFDLEKLRWMNGKYIREVLSLEEVAERVKPFLREAGLSWESEAYLRR
AVELMRPRFDTLKEFPEKARYLFTEDYPVSEKAQRKLEEGLPLLKELYPR
LRAQEEWTEAALEALLRGFAAEKGVKLGQVAQPLRAALTGSLETPGLFEI
LALLGKERALRRLERALA

3D structure

PDB

1g59 Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase.

Chain

Resolution

2.4 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

6.1.1.17: glutamate--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	D44 R45 A46 R47 P109 V145 R163 V166 E172 V177 K180 S181 D182 E207 E208 R237 K241 T242 K243 M273 G274 E282 S299 L300 P303 V304 K309 W312 R319 P357 R358 R417 K426 L427 Q432 R435 L442 E443 T444 P445 G446 L447 F448	D44 R45 A46 R47 P109 V145 R163 V166 E172 V177 K180 S181 D182 E207 E208 R237 K241 T242 K243 M273 G274 E282 S299 L300 P303 V304 K309 W312 R319 P357 R358 R417 K426 L427 Q432 R435 L442 E443 T444 P445 G446 L447 F448	PDBbind-CN: Kd=4.7uM

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004818	glutamate-tRNA ligase activity
GO:0005524	ATP binding
GO:0008270	zinc ion binding

	Biological Process
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006424	glutamyl-tRNA aminoacylation
GO:0043039	tRNA aminoacylation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol

View graph for
Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1g59, PDBe:1g59, PDBj:1g59
PDBsum	1g59
PubMed	11224561
UniProt	P27000\|SYE_THET8 Glutamate--tRNA ligase (Gene Name=gltX)

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