Structure of PDB 1fzh Chain A

Receptor sequence

>1fzhA (length=511) Species: 414 (Methylococcus capsulatus)

NRAPTSVNAQEVHRWLQSFNWDFKNNRTKYATKYKMANETKEQFKLIAKE
YARMEAVKDERQFGSLQDALTRLNAGVRVHPKWNETMKVVSNFLEVGEYN
AIAATGMLWDSAQAAEQKNGYLAQVLDEIRHTHQCAYVNYYFAKNGQDPA
GHNDARRTRTIGPLWKGMKRVFSDGFISGDAVECSLNLQLVGEACFTNPL
IVAVTEWAAANGDEITPTVFLSIETDELRHMANGYQTVVSIANDPASAKY
LNTDLNNAFWTQQKYFTPVLGMLFEYGSKFKVEPWVKTWDRWVYEDWGGI
WIGRLGKYGVESPRSLKDAKQDAYWAHHDLYLLAYALWPTGFFRLALPDQ
EEMEWFEANYPGWYDHYGKIYEEWRARGCEDPSSGFIPLMWFIENNHPIY
IDRVSQVPFCPSLAKGASTLRVHEYNGEMHTFSDQWGERMWLAEPERYEC
QNIFEQYEGRELSEVIAELHGLRSDGKTLIAQPHVRGDKLWTLDDIKRLN
CVFKNPVKAFN

3D structure

• PDB: 1fzh Xenon and halogenated alkanes track putative substrate binding cavities in the soluble methane monooxygenase hydroxylase.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E114 E144 H147 E209 E243 H246
• Catalytic site (residue number reindexed from 1): E98 E128 H131 E193 E227 H230
• Enzyme Commision number: 1.14.13.25: methane monooxygenase (soluble).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE	A	E114 E144 H147	E98 E128 H131
BS02	FE	A	E144 E209 E243 H246	E128 E193 E227 H230
BS03	XE	A	Y115 T148 H149	Y99 T132 H133
BS04	XE	A	L353 L478	L337 L462

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005515 protein binding
• GO:0015049 methane monooxygenase [NAD(P)H] activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006730 one-carbon metabolic process

External links

• PDB: RCSB:1fzh, PDBe:1fzh, PDBj:1fzh
• PDBsum: 1fzh
• PubMed: 11297413
• UniProt: P22869|MEMA_METCA Methane monooxygenase component A alpha chain (Gene Name=mmoX)