Structure of PDB 1fz9 Chain A

Receptor sequence

>1fz9A (length=511) Species: 414 (Methylococcus capsulatus)

NRAPTSVNAQEVHRWLQSFNWDFKNNRTKYATKYKMANETKEQFKLIAKE
YARMEAVKDERQFGSLQDALTRLNAGVRVHPKWNETMKVVSNFLEVGEYN
AIAATGMLWDSAQAAEQKNGYLAQVLDEIRHTHQCAYVNYYFAKNGQDPA
GHNDARRTRTIGPLWKGMKRVFSDGFISGDAVECSLNLQLVGEACFTNPL
IVAVTEWAAANGDEITPTVFLSIETDELRHMANGYQTVVSIANDPASAKY
LNTDLNNAFWTQQKYFTPVLGMLFEYGSKFKVEPWVKTWDRWVYEDWGGI
WIGRLGKYGVESPRSLKDAKQDAYWAHHDLYLLAYALWPTGFFRLALPDQ
EEMEWFEANYPGWYDHYGKIYEEWRARGCEDPSSGFIPLMWFIENNHPIY
IDRVSQVPFCPSLAKGASTLRVHEYNGEMHTFSDQWGERMWLAEPERYEC
QNIFEQYEGRELSEVIAELHGLRSDGKTLIAQPHVRGDKLWTLDDIKRLN
CVFKNPVKAFN

3D structure

• PDB: 1fz9 Xenon and halogenated alkanes track putative substrate binding cavities in the soluble methane monooxygenase hydroxylase.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E114 E144 H147 E209 E243 H246
• Catalytic site (residue number reindexed from 1): E98 E128 H131 E193 E227 H230
• Enzyme Commision number: 1.14.13.25: methane monooxygenase (soluble).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE	A	E114 E144 H147 E243	E98 E128 H131 E227
BS02	FE	A	E144 E209 E243 H246	E128 E193 E227 H230
BS03	ETI	A	T102 L289 G293 L361	T86 L273 G277 L345
BS04	ETI	A	L110 L216	L94 L200

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005515 protein binding
• GO:0015049 methane monooxygenase [NAD(P)H] activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006730 one-carbon metabolic process

External links

• PDB: RCSB:1fz9, PDBe:1fz9, PDBj:1fz9
• PDBsum: 1fz9
• PubMed: 11297413
• UniProt: P22869|MEMA_METCA Methane monooxygenase component A alpha chain (Gene Name=mmoX)