Structure of PDB 1fpm Chain A

Receptor sequence

>1fpmA (length=549) Species: 1525 (Moorella thermoacetica)

DIEIAQAAKMKPVMELARGLGIQEDEVELYGKYKAKISLDVYRRLKDKPD
GKLILVTAITPTPAGEGKTTTSVGLTDALARLGKRVMVCLREPSLGPSFG
IKGGAAGGGYAQVVPMEDINLHFTGDIHAVTYAHNLLAAMVDNHLQQGNV
LNIDPRTITWRRVIDLNDRALRNIVIGLGGKANGVPRETGFDISVASEVM
ACLCLASDLMDLKERFSRIVVGYTYDGKPVTAGDLEAQGSMALLMKDAIK
PNLVQTLENTPAFIHGGPFANIAHGCNSIIATKTALKLADYVVTEAGFGA
DLGAEKFYDVKCRYAGFKPDATVIVATVRALKMHGGVPKSDLATENLEAL
REGFANLEKHIENIGKFGVPAVVAINAFPTDTEAELNLLYELCAKAGAEV
ALSWAKGGEGGLELARKVLQTLESRPSNFHVLYNLDLSIKDKIAKIATEI
YGADGVNYTAEADKAIQRYESLGYGNLPVVMAKTQYSFSDDMTKLGRPRN
FTITVREVRLSAGGRLIVPITGAIMTMPGLPKRPAACNIDIDADGVITG

3D structure

• PDB: 1fpm Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica.
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K74 T76 R97 K108 A276 F304 F384 W412
• Catalytic site (residue number reindexed from 1): K68 T70 R91 K102 A270 F298 F378 W404
• Enzyme Commision number: 6.3.4.3: formate--tetrahydrofolate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CS	A	E98 N126 L127	E92 N120 L121

Gene Ontology

Molecular Function

• GO:0004329 formate-tetrahydrofolate ligase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0035999 tetrahydrofolate interconversion

External links

• PDB: RCSB:1fpm, PDBe:1fpm, PDBj:1fpm
• PDBsum: 1fpm
• PubMed: 11087401
• UniProt: P21164|FTHS_MOOTH Formate--tetrahydrofolate ligase (Gene Name=fhs)