Structure of PDB 1fiu Chain A

Receptor sequence
>1fiuA (length=286) Species: 485 (Neisseria gonorrhoeae) [Search protein sequence]
MQPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIAKGIADL
LHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSR
NRLEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIAD
AEINRNEFLVDENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARS
EGLNLVRNRKGRLPHIVVVTAEPTPSRISSIALGTGEIDCVYHFALYELE
QILQSLNYEDALDLFYIMVNGKRLKDISDLPLDLAV
3D structure
PDB1fiu Structure of the tetrameric restriction endonuclease NgoMIV in complex with cleaved DNA.
ChainA
Resolution1.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A R191 S192 R227 R191 S192 R227
BS02 dna A Q63 T64 R100 D193 Q63 T64 R100 D193
BS03 dna A D34 S36 R59 G62 G66 D140 K187 W188 T189 R191 R194 D34 S36 R59 G62 G66 D140 K187 W188 T189 R191 R194
BS04 MG A D140 C186 D140 C186
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1fiu, PDBe:1fiu, PDBj:1fiu
PDBsum1fiu
PubMed10966652
UniProtP31032|T2M4_NEIGO Type II restriction enzyme NgoMIV (Gene Name=ngoMIVR)

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