Structure of PDB 1fcb Chain A

Receptor sequence

>1fcbA (length=494) Species: 4932 (Saccharomyces cerevisiae)

EPKLDMNKQKISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDVI
KFNAGKDVTAIFEPLHAPNVIDKYIAPEKKLGPLQGSMPPELVCPPYAPG
ETKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTKQAWAYYSSGAND
EVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALC
KLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQW
YQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNG
ASRALSKFIDPSLTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSG
VVLSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTD
VLKALCLGAKGVGLGRPFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGV
TSIAELKPDLLDLSTLKARTVGVPNDVLYNEVYEGPTLTEFEDA

3D structure

• PDB: 1fcb Molecular structure of flavocytochrome b2 at 2.4 A resolution.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S228 Y254 T280 D282 K349 H373
• Catalytic site (residue number reindexed from 1): S228 Y254 T280 D282 K332 H356
• Enzyme Commision number: 1.1.2.3: L-lactate dehydrogenase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	F39 H43 P44 V49 I50 V58 F62 H66 V70 I75 Y97 Y143 L199 L230 K296	F39 H43 P44 V49 I50 V58 F62 H66 V70 I75 Y97 Y143 L199 L230 K296
BS02	FMN	A	Y144 S195 A196 T197 A198 S228 Q252 T280 K349 H373 G374 R376 D409 R413 G432 R433	Y144 S195 A196 T197 A198 S228 Q252 T280 K332 H356 G357 R359 D392 R396 G415 R416

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding

External links

• PDB: RCSB:1fcb, PDBe:1fcb, PDBj:1fcb
• PDBsum: 1fcb
• PubMed: 2329585
• UniProt: P00175|CYB2_YEAST L-lactate dehydrogenase (cytochrome) (Gene Name=CYB2)