Structure of PDB 1f9d Chain A

Receptor sequence

>1f9dA (length=629) Species: 1521 (Ruminiclostridium cellulolyticum)

ASSPANKVYQDRFESMYSKIKDPANGYFSEQGIPYHSIETLMVEAPDYGH
VTTSQAMSYYMWLEAMHGRFSGDFTGFDKSWSVTEQYLIPTEKDQPNTSM
SRYDANKPATYAPEFQDPSKYPSPLDTSQPVGRDPINSQLTSAYGTSMLY
GMHWILDVDNWYGFGARADGTSKPSYINTFQRGEQESTWETIPQPCWDEH
KFGGQYGFLDLFTKDTGTPAKQFKYTNAPDADARAVQATYWADQWAKEQG
KSVSTSVGKATKMGDYLRYSFFDKYFRKIGQPSQAGTGYDAAHYLLSWYY
AWGGGIDSTWSWIIGSSHNHFGYQNPFAAWVLSTDANFKPKSSNGASDWA
KSLDRQLEFYQWLQSAEGAIAGGATNSWNGRYEAVPSGTSTFYGMGYVEN
PVYADPGSNTWFGMQVWSMQRVAELYYKTGDARAKKLLDKWAKWINGEIK
FNADGTFQIPSTIDWEGQPDTWNPTQGYTGNANLHVKVVNYGTDLGCASS
LANTLTYYAAKSGDETSRQNAQKLLDAMWNNYSDSKGISTVEQRGDYHRF
LDQEVFVPAGWTGKMPNGDVIKSGVKFIDIRSKYKQDPEWQTMVAALQAG
QVPTQRLHRFWAQSEFAVANGVYAILFPD

3D structure

• PDB: 1f9d Crystal Structures of the Cellulase Cel48F in Complex with Inhibitors and Substrates Give Insights Into its Processive Action
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.4: cellulase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	W154 F180 T226 N227 W298 Y403	W154 F180 T226 N227 W298 Y403
BS02	GLC	A	N178 F180 T226 Y299 Y403	N178 F180 T226 Y299 Y403
BS03	GLC	A	Q181 K274	Q181 K274
BS04	GLC	A	T110 K224 Y275 W312	T110 K224 Y275 W312
BS05	GLC	A	A109 T110 Q222 W310 W312	A109 T110 Q222 W310 W312
BS06	GLC	A	E542 Q543	E542 Q543
BS07	GLC	A	E44 D494 R609	E44 D494 R609
BS08	GLC	A	H36 E44 Q55 W411 W417 W611	H36 E44 Q55 W411 W417 W611
BS09	GLC	A	T53 Q55 W154 D230 Y323	T53 Q55 W154 D230 Y323
BS10	CA	A	Q185 E190 D405	Q185 E190 D405

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0008810 cellulase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0030245 cellulose catabolic process

External links

• PDB: RCSB:1f9d, PDBe:1f9d, PDBj:1f9d
• PDBsum: 1f9d
• PubMed: 10985769
• UniProt: P37698|GUNF_RUMCH Endoglucanase F (Gene Name=celCCF)