Structure of PDB 1f7u Chain A

Receptor sequence

>1f7uA (length=606) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

ASTANMISQLKKLSIAEPAVAKDSHPDVNIVDLMRNYISQELSKISGVDS
SLIFPALEWTNTMERGDLLIPIPRLRIKGANPKDLAVQWAEKFPCGDFLE
KVEANGPFIQFFFNPQFLAKLVIPDILTRKEDYGSCKLVENKKVIIEFSS
PNIAKPFHAGHLRSTIIGGFLANLYEKLGWEVIRMNYLGDWGKQFGLLAV
GFERYGNEEALVKDPIHHLFDVYVRINKDIEEEGDSIPLEQSTNGKAREY
FKRMEDGDEEALKIWKRFREFSIEKYIDTYARLNIKYDVYSGESQVSKES
MLKAIDLFKEKGLTHEDKGAVLIDLTKFNKKLGKAIVQKSDGTTLYLTRD
VGAAMDRYEKYHFDKMIYVIASQQDLHAAQFFEILKQMGFEWAKDLQHVN
FGMVQGMSTRKGTVVFLDNILEETKEKMHEVMKKNENKYAQIEHPEEVAD
LVGISAVMIQDMQGKRINNYEFKWERMLSFEGDTGPYLQYAHSRLRSVER
NASGITQEKWINADFSLLKEPAAKLLIRLLGQYPDVLRNAIKTHEPTTVV
TYLFKLTHQVSSCYDVLWVAGQTEELATARLALYGAARQVLYNGMRLLGL
TPVERM

3D structure

PDB

1f7u tRNA aminoacylation by arginyl-tRNA synthetase: induced conformations during substrates binding

Chain

Resolution

2.2 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	K156 H159 H162
Catalytic site (residue number reindexed from 1)	K155 H158 H161
Enzyme Commision number	6.1.1.19: arginine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E59 W60 R66 P72 P74 R75 R77 N106 F109 Q111 N153 E294 K319 K332 I337 K340 S341 L346 Y347 R350 Q374 Q375 L377 M404 Q406 V432 K439 K466 N469 F481 G483 D484 Y488 Y491 R495 S498 N502 S562 S563 Y565 D566 L568 W569 V570 A571 M607	E58 W59 R65 P71 P73 R74 R76 N105 F108 Q110 N152 E293 K318 K331 I336 K339 S340 L345 Y346 R349 Q373 Q374 L376 M403 Q405 V431 K438 K465 N468 F480 G482 D483 Y487 Y490 R494 S497 N501 S561 S562 Y564 D565 L567 W568 V569 A570 M606
BS02	ARG	A	E148 S151 N153 Y188 Y347 D351 Y369 I371	E147 S150 N152 Y187 Y346 D350 Y368 I370

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004814	arginine-tRNA ligase activity
GO:0005524	ATP binding
GO:1990825	sequence-specific mRNA binding

	Biological Process
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006420	arginyl-tRNA aminoacylation
GO:0032543	mitochondrial translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1f7u, PDBe:1f7u, PDBj:1f7u
PDBsum	1f7u
PubMed	11060012
UniProt	Q05506\|SYRC_YEAST Arginine--tRNA ligase, cytoplasmic (Gene Name=YDR341C)

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