Structure of PDB 1eyu Chain A

Receptor sequence
>1eyuA (length=156) Species: 585 (Proteus vulgaris) [Search protein sequence]
SHPDLNKLLELWPHIQEYQDLALKHGINDIFQDNGGKLLQVLLITGLTVL
PGREGNDAVDNAGQEYELKSINIDLTKGFSTHHHMNPVIIAKYRQVPWIF
AIYRGIAIEAIYRLEPKDLEFYYDKWERKWYSDGHKDINNPKIPVKYVME
HGTKIY
3D structure
PDB1eyu PvuII endonuclease contains two calcium ions in active sites.
ChainA
Resolution1.78 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D58 E68 K70
Catalytic site (residue number reindexed from 1) D57 E67 K69
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A Q33 H84 K130 N140 N141 Q32 H83 K129 N139 N140
BS02 dna A D34 N35 E55 N57 K70 S71 N73 L76 S81 T82 H83 H84 K93 N141 K143 D33 N34 E54 N56 K69 S70 N72 L75 S80 T81 H82 H83 K92 N140 K142
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1eyu, PDBe:1eyu, PDBj:1eyu
PDBsum1eyu
PubMed10903853
UniProtP23657|T2P2_PROHU Type II restriction enzyme PvuII (Gene Name=pvuIIR)

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