Structure of PDB 1ej9 Chain A

Receptor sequence

>1ej9A (length=483) Species: 9606 (Homo sapiens)

WKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPK
AEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDF
TQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIAN
FKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWK
EVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKK
CVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTV
GCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQ
LFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQL
KELTAPDENIPAKILSYNRANRAVAILCNHQIALGTSKLNFLDPRITVAW
CKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

3D structure

• PDB: 1ej9 Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	H266 R364 V410 T411 W412 I424 Y426 M428 K436 K443 R488 K532 D533 I535 K587 H632 Q633 T718 F723	H64 R162 V208 T209 W210 I222 Y224 M226 K234 K241 R286 K330 D331 I333 K385 H430 Q431 T436 F441
BS02	dna	A	E356 P357 R362 G363 R364 H367 K425 A489 G490 N491 K493 T501 K532 D533 T585 A586 K587 N745 T747	E154 P155 R160 G161 R162 H165 K223 A287 G288 N289 K291 T299 K330 D331 T383 A384 K385 N463 T465

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1ej9, PDBe:1ej9, PDBj:1ej9
• PDBsum: 1ej9
• PubMed: 10841763
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)