Structure of PDB 1ehi Chain A

Receptor sequence
>1ehiA (length=360) Species: 1245 (Leuconostoc mesenteroides) [Search protein sequence]
KKRVALIFGGNSSEHDVSKRSAQNFYNAIEATGKYEIIVFAIAQNGFFLD
TESSKKILALEDEQPIVDAFMKTVDASDPLARIHALKSAGDFDIFFPVVH
GNLGEDGTLQGLFKLLDKPYVGAPLRGHAVSFDKALTKELLTVNGIRNTK
YIVVDPESANNWSWDKIVAELGNIVFVKAANQGSSVGISRVTNAEEYTEA
LSDSFQYDYKVLIEEAVNGARELEVGVIGNDQPLVSEIGAHTVPNQGSGD
GWYDYNNKFVDNSAVHFQIPAQLSPEVTKEVKQMALDAYKVLNLRGEARM
DFLLDENNVPYLGEPNTLPGFTNMSLFKRLWDYSDINNAKLVDMLIDYGF
EDFAQNKKLS
3D structure
PDB1ehi Enzymes of vancomycin resistance: the structure of D-alanine-D-lactate ligase of naturally resistant Leuconostoc mesenteroides.
ChainA
Resolution2.38 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S186 N258 F261 N264 R301 D303 E316 N318 G322
Catalytic site (residue number reindexed from 1) S184 N256 F259 N262 R299 D301 E314 N316 G320
Enzyme Commision number 6.3.2.4: D-alanine--D-alanine ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A E316 N318 E314 N316
BS02 ADP A F178 K180 G185 S186 S187 I190 E216 A218 V219 E224 W254 Y255 L305 E316 F176 K178 G183 S184 S185 I188 E214 A216 V217 E222 W252 Y253 L303 E314
BS03 PHY A E16 K260 R301 N318 G322 M326 S327 E14 K258 R299 N316 G320 M324 S325
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0005524 ATP binding
GO:0008716 D-alanine-D-alanine ligase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0008360 regulation of cell shape
GO:0009252 peptidoglycan biosynthetic process
GO:0071555 cell wall organization
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:1ehi, PDBe:1ehi, PDBj:1ehi
PDBsum1ehi
PubMed10801495
UniProtQ03ZI1|DDL_LEUMM D-alanine--D-alanine ligase (Gene Name=ddl)

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