Structure of PDB 1eam Chain A

Receptor sequence
>1eamA (length=285) Species: 10245 (Vaccinia virus) [Search protein sequence]
MDVVSLDKPFMYFEEIDNELDYEPAKKLPYQGQLKLLLGELFFLSKLQRH
GILDGATVVYIGSAPGTHIRYLRDHFYNLGVIIKWMLIDGRHHDPILNGL
RDVTLVTRFVDEEYLRSIKKQLHPSKIILISDVRSPSTADLLSNYALQNV
MISILNPVASSLKWRCPFPDQWIKDFYIPHGNKMLQPFAPSYSAEMRLLS
IYTGENMRLTRVTKSDAVNYAKKMYYLNKIVRNKVVVNFDYPNQEYDYFH
MYFMLRTVYCNKTFPTTKAKVLFLQQSIFRFLNIP
3D structure
PDB1eam mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.57: methyltransferase cap1.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A Q39 L42 Y66 G68 S69 A70 P71 G72 H74 D95 R97 F115 V116 D138 R140 Q33 L36 Y60 G62 S63 A64 P65 G66 H68 D89 R91 F109 V110 D132 R134
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003746 translation elongation factor activity
GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
GO:0008168 methyltransferase activity
Biological Process
GO:0006370 7-methylguanosine mRNA capping
GO:0006412 translation
GO:0006414 translational elongation
GO:0031440 regulation of mRNA 3'-end processing
GO:0032259 methylation
Cellular Component
GO:0044423 virion component

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1eam, PDBe:1eam, PDBj:1eam
PDBsum1eam
PubMed10377383
UniProtP07617|MCE_VACCW Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase (Gene Name=OPG102)

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