Structure of PDB 1e55 Chain A

Receptor sequence
>1e55A (length=492) Species: 4577 (Zea mays) [Search protein sequence]
VQMLSPSEIPQRDWFPSDFTFGAATSAYQIEGAWNEDGKGESNWDHFCHN
HPERILDGSNSDIGANSYHMYKTDVRLLKEMGMDAYRFSISWPRILPKGT
KEGGINPDGIKYYRNLINLLLENGIEPYVTIFHWDVPQALEEKYGGFLDK
SHKSIVEDYTYFAKVCFDNFGDKVKNWLTFNDPQTFTSFSYGTGVFAPGR
CSPGLDCAYPTGNSLVEPYTAGHNILLAHAEAVDLYNKHYKRDDTRIGLA
FDVMGRVPYGTSFLDKQAEERSWDINLGWFLEPVVRGDYPFSMRSLARER
LPFFKDEQKEKLAGSYNMLGLNYYTSRFSKNIDISPNYSPVLNTDDAYAS
QEVNGPDGKPIGPPMGNPWIYMYPEGLKDLLMIMKNKYGNPPIYITENGI
GDVDTKETPLPMEAALNDYKRLDYIQRHIATLKESIDLGSNVQGYFAWSL
LDNFEWFAGFTERYGIVYVDRNNNCTRYMKESAKWLKEFNTA
3D structure
PDB1e55 The mechanism of substrate (aglycone) specificity in beta-glucosidases is revealed by crystal structures of mutant maize beta-glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes.
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R96 H142 D191 T194 N331 Y333 E406
Catalytic site (residue number reindexed from 1) R87 H133 D182 T185 N322 Y324 E397
Enzyme Commision number 3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase.
3.2.1.21: beta-glucosidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 BGC A D191 Y333 E406 W457 E464 Y473 D182 Y324 E397 W448 E455 Y464 PDBbind-CN: -logKd/Ki=4.12,Ki=76uM
BS02 DHR A F198 F466 F189 F457 MOAD: Ki=76uM
PDBbind-CN: -logKd/Ki=4.12,Ki=76uM
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0008422 beta-glucosidase activity
GO:0015923 mannosidase activity
GO:0015925 galactosidase activity
GO:0015928 fucosidase activity
GO:0016162 cellulose 1,4-beta-cellobiosidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0097599 xylanase activity
GO:0102726 DIMBOA glucoside beta-D-glucosidase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0009736 cytokinin-activated signaling pathway
Cellular Component
GO:0009507 chloroplast

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1e55, PDBe:1e55, PDBj:1e55
PDBsum1e55
PubMed11106394
UniProtP49235|HGGL1_MAIZE 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 1, chloroplastic (Gene Name=GLU1)

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