Structure of PDB 1e4e Chain A

Receptor sequence
>1e4eA (length=341) Species: 1352 (Enterococcus faecium) [Search protein sequence]
NRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGITKSGVWKM
CEKPCAEWENENCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGK
SGEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFW
VINKDDRPVAATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYD
SKILIEQAVSGCEVGCAVLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEK
GSENAVITVPADLSAEERGRIQETVKKIYKTLGCRGLARVDMFLQDNGRI
VLNEVNTLPGFTSYSRYPRMMAAAGISLPELIDRLIVLALK
3D structure
PDB1e4e The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).
ChainA
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E16 V19 K22 H99 S177 H244 V247 E250 R290 D292 E305 N307 G311 Y315
Catalytic site (residue number reindexed from 1) E15 V18 K21 H98 S176 H243 V246 E249 R289 D291 E304 N306 G310 Y314
Enzyme Commision number 6.1.2.1: D-alanine--(R)-lactate ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP A K133 F169 K171 G176 S178 E207 V210 E214 F241 F294 N304 E305 K132 F168 K170 G175 S177 E206 V209 E213 F240 F293 N303 E304
BS02 PHY A E16 S177 H244 R290 E305 N307 G311 S316 R317 E15 S176 H243 R289 E304 N306 G310 S315 R316
BS03 MG A E305 N307 E304 N306
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0005524 ATP binding
GO:0008716 D-alanine-D-alanine ligase activity
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0008360 regulation of cell shape
GO:0009252 peptidoglycan biosynthetic process
GO:0046677 response to antibiotic
GO:0071555 cell wall organization
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1e4e, PDBe:1e4e, PDBj:1e4e
PDBsum1e4e
PubMed10908650
UniProtP25051|VANA_ENTFC Vancomycin/teicoplanin A-type resistance protein VanA (Gene Name=vanA)

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