Structure of PDB 1e3m Chain A

Receptor sequence

>1e3mA (length=788) Species: 562 (Escherichia coli)

SAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDAKRASQLL
DISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPAT
SKGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDIS
SGRFRLSEPADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPL
WEFEIDTARQQLNLQFGTRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTT
LPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVTP
MGSRMLKRWLHMPVRDTRVLLERQQTIGALQDFTAGLQPVLRQVGDLERI
LARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFA
ELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERLE
VRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERY
IIPELKEYEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAE
LDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPL
NLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPID
RIFTRVGFMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLSLAWACA
ENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSV
QDGAASKSYGLAVAALAGVPKEVIKRARQKLRELESIS

3D structure

• PDB: 1e3m The Crystal Structure of DNA Mismatch Repair Protein Muts Binding to a G X T Mismatch
• Chain: A
• Resolution: 2.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P12 M13 M33 G34 D35 F36 R58 A60 S61 Q95 P99 P105 V106 R108 V470	P11 M12 M32 G33 D34 F35 R57 A59 S60 Q94 P98 P104 V105 R107 V469
BS02	dna	A	F36 E38 I53 S54 T56 M68 G70 P72 Y79 N468 L495 K496 R500	F35 E37 I52 S53 T55 M67 G69 P71 Y78 N467 L494 K495 R499
BS03	ADP	A	F596 I597 M617 G619 K620 S621 T622 H760	F595 I596 M616 G618 K619 S620 T621 H748
BS04	MG	A	P99 S102	P98 S101

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008301 DNA binding, bending
• GO:0016887 ATP hydrolysis activity
• GO:0030983 mismatched DNA binding
• GO:0032136 adenine/cytosine mispair binding
• GO:0042802 identical protein binding
• GO:0043531 ADP binding
• GO:0140664 ATP-dependent DNA damage sensor activity

Biological Process

• GO:0000018 regulation of DNA recombination
• GO:0006281 DNA repair
• GO:0006298 mismatch repair
• GO:0006974 DNA damage response

Cellular Component

• GO:0005829 cytosol
• GO:0032300 mismatch repair complex
• GO:1990710 MutS complex

External links

• PDB: RCSB:1e3m, PDBe:1e3m, PDBj:1e3m
• PDBsum: 1e3m
• PubMed: 11048711
• UniProt: P23909|MUTS_ECOLI DNA mismatch repair protein MutS (Gene Name=mutS)