Structure of PDB 1dmu Chain A

Receptor sequence
>1dmuA (length=299) Species: 1423 (Bacillus subtilis) [Search protein sequence]
MYNLHREKIFMSYNQNKQYLEDNPEIQEKIELYGLNLLNEVISDNEEEIR
ADYNEANFLHPFWMNYPPLDRGKMPKGDQIPWIEVGEKAVGSKLTRLVSQ
REDITVREIGLPTGPDERYLLTSPTIYSLTNGFTDSIMMFVDIKSVGPRD
SDYDLVLSPNQVSGNGDWAQLEGGIQNNQQTIQGPRSSQIFLPTIPPLYI
LSDGTIAPVVHLFIKPIYAMRSLTKGDTGQSLYKIKLASVPNGLGLFCNP
GYAFDSAYKFLFRPGKDDRTKSLLQKRVRVDLRVLDKIGPRVMTIDMDK
3D structure
PDB1dmu Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence.
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D116 D142 I143 K144
Catalytic site (residue number reindexed from 1) D116 D142 I143 K144
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K73 M74 K76 Q79 I83 D116 S145 V146 G147 R149 D150 N160 Y218 K266 R277 K73 M74 K76 Q79 I83 D116 S145 V146 G147 R149 D150 N160 Y218 K266 R277
BS02 CA A D116 D142 I143 D116 D142 I143
BS03 CA A S99 Q100 R101 E102 I104 S99 Q100 R101 E102 I104
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1dmu, PDBe:1dmu, PDBj:1dmu
PDBsum1dmu
PubMed9736624
UniProtO68557|T2B1_BACIU Type II restriction enzyme BglI (Gene Name=bglIR)

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