Structure of PDB 1dig Chain A

Receptor sequence
>1digA (length=285) Species: 9606 (Homo sapiens) [Search protein sequence]
APAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNL
YINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQL
PLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPKG
CLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAH
LDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYKVVGDVAYDEA
KERASFITPVPGGVGPMTVAMLMQSTVESAKRFLE
3D structure
PDB1dig Structures of three inhibitor complexes provide insight into the reaction mechanism of the human methylenetetrahydrofolate dehydrogenase/cyclohydrolase.
ChainA
Resolution2.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP(+)).
3.5.4.9: methenyltetrahydrofolate cyclohydrolase.
6.3.4.3: formate--tetrahydrofolate ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAP A T148 R173 S174 V177 H196 S197 L202 A215 T216 M221 C236 I238 G276 T279 T147 R172 S173 V176 H195 S196 L201 A214 T215 M220 C235 I237 G265 T268
BS02 L37 A K56 G273 G276 P277 K55 G262 G265 P266 MOAD: Ki=431uM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity

View graph for
Molecular Function
External links
PDB RCSB:1dig, PDBe:1dig, PDBj:1dig
PDBsum1dig
PubMed10828945
UniProtP11586|C1TC_HUMAN C-1-tetrahydrofolate synthase, cytoplasmic (Gene Name=MTHFD1)

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