Structure of PDB 1dfm Chain A

Receptor sequence
>1dfmA (length=223) Species: 1423 (Bacillus subtilis) [Search protein sequence]
MKIDITDYNHADEILNPQLWKEIEETLLKMPLHVKASDQASKVGSLIFDP
VGTNQYIKDELVPKHWKNNIPIPKRFDFLGTDIDFGKRDTLVEVQFSNYP
FLLNNTVRSELFHKSNMDIDEEGMKVAIIITKGHMFPASNSSLYYEQAQN
QLNSLAEYNVFDVPIRLVGLIEDFETDIDIVSTTYADKRYSRTITKRDTV
KGKVIDTNTPNTRRRKRGTIVTY
3D structure
PDB1dfm Understanding the immutability of restriction enzymes: crystal structure of BglII and its DNA substrate at 1.5 A resolution.
ChainA
Resolution1.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K35 D38 Q39 A40 P50 V51 N54 G80 T81 D82 D84 S97 N98 R108 N140 R189 Y190 K35 D38 Q39 A40 P50 V51 N54 G80 T81 D82 D84 S97 N98 R108 N140 R189 Y190
BS02 dna A Y99 H134 N140 S141 Y144 Y190 R192 R215 R217 Y99 H134 N140 S141 Y144 Y190 R192 R215 R217
BS03 CA A D84 V94 D84 V94
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1dfm, PDBe:1dfm, PDBj:1dfm
PDBsum1dfm
PubMed10655616
UniProtQ45488|T2B2_BACIU Type II restriction enzyme BglII (Gene Name=bglIIR)

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