Structure of PDB 1d4d Chain A

Receptor sequence

>1d4dA (length=560) Species: 70863 (Shewanella oneidensis)

VLADFHGEMGGCDSCHVSDKGGVTNDNLTHENGQCVSCHGDLKELAAAAP
VSPHKSHLIGEIACTSCHKGHEKSVAYCDACHSFGFDMPFGGKWERKFVP
VDADKAAQDKAIAAGVKETTDVVIIGSGGAGLAAAVSARDAGAKVILLEK
EPIPGGNTKLAAGGMNAAETKPQAKLGIEDKKQIMIDDTMKGGRNINDPE
LVKVLANNSSDSIDWLTSMGADMTDVGRMGGASVNRSHRPTGGAGVGAHV
AQVLWDNAVKRGTDIRLNSRVVRILEDGKVTGVLVKGEYTGYYVIKADAV
VIAAGGFAKNNERVSKYDPKLKGFKATNHPGATGDGLDVALQAGAATRDL
QYIQAHPTYSPAGGVMITEAVRGNGAIVVNREGNRFMNEITTRDKASAAI
LQQKGESAYLVFDDSIRKSLKAIEGYVHLNIVKEGKTIEELAKQIDVPAA
ELAKTVTAYNGFVSGKDAQFERPDLPRELVVAPFYALEIAPAVHHTMGGL
VIDTKAEVKSEKTAKPITGLYAAGEVTGGVHGANRLGGNAISDIVTYGRI
AGASAAKFAK

3D structure

• PDB: 1d4d Structure and mechanism of the flavocytochrome c fumarate reductase of Shewanella putrefaciens MR-1.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): M235 Q362 H364 M374 E377 R380 R401 H503 H504 R544
• Catalytic site (residue number reindexed from 1): M229 Q354 H356 M366 E369 R372 R393 H494 H495 R535
• Enzyme Commision number: 1.3.2.4: fumarate reductase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	S62 H63 L64 Y83 C84 C87 H88 F90 L166 H337 V373 G433 Y434 L437	S56 H57 L58 Y77 C78 C81 H82 F84 L160 H329 V365 G425 Y426 L429
BS02	HEC	A	H42 L45 L48 P59 H60 I68 C70 C73 H74 M94	H39 L42 L45 P53 H54 I62 C64 C67 H68 M88
BS03	HEC	A	A6 H9 C15 C18 H19 K23 G24 G25 K75 G76 H77 Y297	A3 H6 C12 C15 H16 K20 G21 G22 K69 G70 H71 Y289
BS04	HEC	A	L5 F8 H9 M12 Q37 C38 C41 H42 H74 P95	L2 F5 H6 M9 Q34 C35 C38 H39 H68 P89
BS05	FAD	A	G132 G134 A136 L154 E155 K156 G162 N163 T164 A167 A168 G169 G170 R276 V277 A312 G313 T335 H337 D343 M374 H503 H504 G533 E534 R544 G547 A549 I550 I553	G126 G128 A130 L148 E149 K150 G156 N157 T158 A161 A162 G163 G164 R270 V271 A304 G305 T327 H329 D335 M366 H494 H495 G524 E525 R535 G538 A540 I541 I544

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity

External links

• PDB: RCSB:1d4d, PDBe:1d4d, PDBj:1d4d
• PDBsum: 1d4d
• PubMed: 10581551
• UniProt: P83223|FCCA_SHEON Fumarate reductase (cytochrome) (Gene Name=fccA)