Structure of PDB 1cxk Chain A

Receptor sequence

>1cxkA (length=686) Species: 1397 (Niallia circulans) [Search protein sequence]

APDTSVSNKQNFSTDVIYQIFTDRFSDGNPANNPTGAAFDGTCTNLRLYC
GGDWQGIINKINDGYLTGMGVTAIWISQPVENIYSIINYSGVNNTAYHGY
WARDFKKTNPAYGTIADFQNLIAAAHAKNIKVIIDFAPNHTSPASSDQPS
FAENGRLYDNGTLLGGYTNDTQNLFHHNGGTDFSTTENGIYKNLYDLADL
NHNNSTVDVYLKDAIKMWLDLGIDGIRMNAVKHMPFGWQKSFMAAVNNYK
PVFTFGQWFLGVNEVSPENHKFANESGMSLLDFRFAQKVRQVFRDNTDNM
YGLKAMLEGSAADYAQVDDQVTFIDNHDMERFHASNANRRKLEQALAFTL
TSRGVPAIYYGTEQYMSGGTDPDNRARIPSFSTSTTAYQVIQKLAPLRKC
NPAIAYGSTQERWINNDVLIYERKFGSNVAVVAVNRNLNAPASISGLVTS
LPQGSYNDVLGGLLNGNTLSVGSGGAASNFTLAAGGTAVWQYTAATATPT
IGHVGPMMAKPGVTITIDGRGFGSSKGTVYFGTTAVSGADITSWEDTQIK
VKIPAVAGGNYNIKVANAAGTASNVYDNFEVLSGDQVSVRFVVNNATTAL
GQNVYLTGSVSELGNWDPAKAIGPMYNQVVYQYPNWYYDVSVPAGKTIEF
KFLKKQGSTVTWEGGSNHTFTAPSSGTATINVNWQP

3D structure

PDB

1cxk X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.

Chain

Resolution

2.09 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	D135 R227 N229 Q257 H327 D328
Catalytic site (residue number reindexed from 1)	D135 R227 N229 Q257 H327 D328
Enzyme Commision number	2.4.1.19: cyclomaltodextrin glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLC	A	W413 I414 G446	W413 I414 G446
BS02	GLC	A	E411 R412	E411 R412
BS03	GLC	A	K232 F259	K232 F259
BS04	GLC	A	Y195 A230 H233 Q257	Y195 A230 H233 Q257
BS05	GLC	A	H140 N229 Q257 H327 D328	H140 N229 Q257 H327 D328
BS06	GLC	A	Y100 W101 Y195 D371 R375	Y100 W101 Y195 D371 R375
BS07	GLC	A	Y89 Y195 D196 D371	Y89 Y195 D196 D371
BS08	GLC	A	G180 N193 Y195	G180 N193 Y195
BS09	GLC	A	S145 S146 D147	S145 S146 D147
BS10	GLC	A	K651 W662	K651 W662
BS11	GLC	A	W616 W662 N667	W616 W662 N667
BS12	GLC	A	T598 A599 L600 Y633	T598 A599 L600 Y633
BS13	GLC	A	L600 G601 Q602 N603 N627	L600 G601 Q602 N603 N627
BS14	CA	A	D27 N29 P30 N32 N33 G51 D53	D27 N29 P30 N32 N33 G51 D53
BS15	CA	A	I190 D199 H233	I190 D199 H233

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0004556	alpha-amylase activity
GO:0016757	glycosyltransferase activity
GO:0030246	carbohydrate binding
GO:0043169	cation binding
GO:0043895	cyclomaltodextrin glucanotransferase activity
GO:0046872	metal ion binding
GO:2001070	starch binding

	Biological Process
GO:0005975	carbohydrate metabolic process

	Cellular Component
GO:0005576	extracellular region

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1cxk, PDBe:1cxk, PDBj:1cxk
PDBsum	1cxk
PubMed	10331869
UniProt	P43379\|CDGT2_NIACI Cyclomaltodextrin glucanotransferase (Gene Name=cgt)

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