Structure of PDB 1cxf Chain A

Receptor sequence

>1cxfA (length=686) Species: 1397 (Niallia circulans) [Search protein sequence]

APDTSVSNKQNFSTDVIYQIFTDRFSDGNPANNPTGAAFDGTCTNLRLYC
GGDWQGIINKINDGYLTGMGVTAIWISQPVENIYSIINYSGVNNTAYHGY
WARDFKKTNPAYGTIADFQNLIAAAHAKNIKVIIDFAPNHTSPASSDQPS
FAENGRLYDNGTLLGGYTNDTQNLFHHNGGTDFSTTENGIYKNLYDLADL
NHNNSTVDVYLKDAIKMWLDLGIDGIRMNAVKHMPFGWQKSFMAAVNNYK
PVFTFGQWFLGVNEVSPENHKFANESGMSLLDFRFAQKVRQVFRDNTDNM
YGLKAMLEGSAADYAQVDDQVTFIDNHDMERFHASNANRRKLEQALAFTL
TSRGVPAIYYGTEQYMSGGTDPDNRARIPSFSTSTTAYQVIQKLAPLRKC
NPAIAYGSTQERWINNDVLIYERKFGSNVAVVAVNRNLNAPASISGLVTS
LPQGSYNDVLGGLLNGNTLSVGSGGAASNFTLAAGGTAVWQYTAATATPT
IGHVGPMMAKPGVTITIDGRGFGSSKGTVYFGTTAVSGADITSWEDTQIK
VKIPAVAGGNYNIKVANAAGTASNVYDNFEVLSGDQVSVRFVVNNATTAL
GQNVYLTGSVSELGNWDPAKAIGPMYNQVVYQYPNWYYDVSVPAGKTIEF
KFLKKQGSTVTWEGGSNHTFTAPSSGTATINVNWQP

3D structure

PDB

1cxf Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products.

Chain

Resolution

2.1 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	D135 R227 N229 Q257 H327 D328
Catalytic site (residue number reindexed from 1)	D135 R227 N229 Q257 H327 D328
Enzyme Commision number	2.4.1.19: cyclomaltodextrin glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLC	A	Y195 A230 H233 Q257	Y195 A230 H233 Q257
BS02	GLC	A	H140 N229 Q257 H327 D328	H140 N229 Q257 H327 D328
BS03	GLC	A	L197 D371 R375	L197 D371 R375
BS04	GLC	A	W413 I414 S445	W413 I414 S445
BS05	GLC	A	E411 R412 G446	E411 R412 G446
BS06	GLC	A	K651 W662	K651 W662
BS07	GLC	A	W616 W662 N667	W616 W662 N667
BS08	GLC	A	T598 A599 L600	T598 A599 L600
BS09	GLC	A	A599 L600 G601 Q602 N603 N627 W636	A599 L600 G601 Q602 N603 N627 W636
BS10	CA	A	D27 N29 N32 N33 G51 D53	D27 N29 N32 N33 G51 D53
BS11	CA	A	N139 I190 D199 H233	N139 I190 D199 H233

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0004556	alpha-amylase activity
GO:0016757	glycosyltransferase activity
GO:0030246	carbohydrate binding
GO:0043169	cation binding
GO:0043895	cyclomaltodextrin glucanotransferase activity
GO:0046872	metal ion binding
GO:2001070	starch binding

	Biological Process
GO:0005975	carbohydrate metabolic process

	Cellular Component
GO:0005576	extracellular region

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1cxf, PDBe:1cxf, PDBj:1cxf
PDBsum	1cxf
PubMed	7493956
UniProt	P43379\|CDGT2_NIACI Cyclomaltodextrin glucanotransferase (Gene Name=cgt)

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