Structure of PDB 1cqj Chain A

Receptor sequence

>1cqjA (length=286) Species: 562 (Escherichia coli)

SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGTTHLGL
PVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIP
TLDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIV
SRSGTLTYEAVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQT
EAIVMIGEIGGSAEEEAAAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAI
IAGGKGTADEKFAALEAAGVKTVRSLADIGEALKTV

3D structure

• PDB: 1cqj ADP-binding site of Escherichia coli succinyl-CoA synthetase revealed by x-ray crystallography.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E208 H246
• Catalytic site (residue number reindexed from 1): E208 H246
• Enzyme Commision number: 6.2.1.5: succinate--CoA ligase (ADP-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO4	A	S153 G154 T155 H246	S153 G154 T155 H246
BS02	COA	A	T16 G17 S18 Q19 P40 K42 V72 P73 T96 E97 N122 C123 P124	T16 G17 S18 Q19 P40 K42 V72 P73 T96 E97 N122 C123 P124

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004775 succinate-CoA ligase (ADP-forming) activity
• GO:0004776 succinate-CoA ligase (GDP-forming) activity
• GO:0005515 protein binding
• GO:0016874 ligase activity

Biological Process

• GO:0006099 tricarboxylic acid cycle

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0009361 succinate-CoA ligase complex (ADP-forming)

External links

• PDB: RCSB:1cqj, PDBe:1cqj, PDBj:1cqj
• PDBsum: 1cqj
• PubMed: 10625475
• UniProt: P0AGE9|SUCD_ECOLI Succinate--CoA ligase [ADP-forming] subunit alpha (Gene Name=sucD)