Structure of PDB 1ckn Chain A

Receptor sequence
>1cknA (length=317) Species: 10506 (Paramecium bursaria Chlorella virus 1) [Search protein sequence]
NITTERAVLTLNGLQIKLHKVVGESRDDIVAKMKDLAMDDHKFPRLPGPN
PVSIERKDFEKLKQNKYVVSEKTDGIRFMMFFTRVFGFKVCTIIDRAMTV
YLLPFKNIPRVLFQGSIFDGELCVDIVEKKFAFVLFDAVVVSGVTVSQMD
LASRFFAMKRSLKEFKNVPEDPAILRYKEWIPLEHPTIIKDHLKKANAIY
HTDGLIIMSVDEPVIYGRNFNLFKLKPGTHHTIDFIIMSEDGTIGIFDPN
LRKNVPVGKLDGYYNKGSIVECGFADGTWKYIQGRSDKNQANDRLTYEKT
LLNIEENITIDELLDLF
3D structure
PDB1ckn X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes.
ChainA
Resolution2.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.50: mRNA guanylyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GTP A P61 K82 T83 R87 E131 F146 K188 W190 I216 K234 P51 K72 T73 R77 E121 F136 K178 W180 I206 K224
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004484 mRNA guanylyltransferase activity
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0016779 nucleotidyltransferase activity
Biological Process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006370 7-methylguanosine mRNA capping

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1ckn, PDBe:1ckn, PDBj:1ckn
PDBsum1ckn
PubMed9160746
UniProtQ84424|MCE_PBCV1 mRNA-capping enzyme (Gene Name=A103R)

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