Structure of PDB 1cjy Chain A

Receptor sequence
>1cjyA (length=633) Species: 9606 (Homo sapiens) [Search protein sequence]
EHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRH
FNNDINPVWNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSM
KVGEKKEVPFIFNQVTEMVLEMSLEVCSCPDLRFSMALCDQEKTFRQQRK
EHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMKAL
YESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHN
PLLLLTPQKVKRYVESLWKKKSSGQPVTFTDIFGMLIGETLIHNRMNTTL
SSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFSPYEIGMAKYGTF
MAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLSTMEEEL
ENITTKHIVSNNQASWIHRMIMALVSDSALFNTREGRAGKVHNFMLGLNL
DVKSKKIHVVDSGLTFNLPYPLILRPQRGVDLIISFDFSARPSDSSPPFK
ELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDMEKDCPTII
HFVLANINFRKYKAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQ
AFKRLHDLMHFNTLNNIDVIKEAMVESIEYRRQ
3D structure
PDB1cjy Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism.
ChainA
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G197 G198 R200 S228 D549
Catalytic site (residue number reindexed from 1) G185 G186 R188 S216 D461
Enzyme Commision number 3.1.1.4: phospholipase A2.
3.1.1.5: lysophospholipase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA A D40 T41 D43 N65 D28 T29 D31 N53
BS02 CA A D40 D43 D93 A94 N95 D28 D31 D81 A82 N83
Gene Ontology
Molecular Function
GO:0004620 phospholipase activity
GO:0004622 lysophospholipase activity
GO:0004623 phospholipase A2 activity
GO:0005509 calcium ion binding
GO:0005544 calcium-dependent phospholipid binding
GO:0008289 lipid binding
GO:0008374 O-acyltransferase activity
GO:0010314 phosphatidylinositol-5-phosphate binding
GO:0016787 hydrolase activity
GO:0032266 phosphatidylinositol-3-phosphate binding
GO:0046872 metal ion binding
GO:0047498 calcium-dependent phospholipase A2 activity
GO:0047499 calcium-independent phospholipase A2 activity
GO:0070273 phosphatidylinositol-4-phosphate binding
GO:1902387 ceramide 1-phosphate binding
Biological Process
GO:0001516 prostaglandin biosynthetic process
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0006071 glycerol metabolic process
GO:0006640 monoacylglycerol biosynthetic process
GO:0006650 glycerophospholipid metabolic process
GO:0006663 platelet activating factor biosynthetic process
GO:0006690 icosanoid metabolic process
GO:0009395 phospholipid catabolic process
GO:0010572 positive regulation of platelet activation
GO:0019369 arachidonate metabolic process
GO:0019370 leukotriene biosynthetic process
GO:0032308 positive regulation of prostaglandin secretion
GO:0034478 phosphatidylglycerol catabolic process
GO:0034638 phosphatidylcholine catabolic process
GO:0036151 phosphatidylcholine acyl-chain remodeling
GO:0042127 regulation of cell population proliferation
GO:0043032 positive regulation of macrophage activation
GO:0046456 icosanoid biosynthetic process
GO:0046475 glycerophospholipid catabolic process
GO:0050482 arachidonate secretion
GO:0051649 establishment of localization in cell
GO:0071236 cellular response to antibiotic
Cellular Component
GO:0000139 Golgi membrane
GO:0005634 nucleus
GO:0005635 nuclear envelope
GO:0005737 cytoplasm
GO:0005743 mitochondrial inner membrane
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1cjy, PDBe:1cjy, PDBj:1cjy
PDBsum1cjy
PubMed10319815
UniProtP47712|PA24A_HUMAN Cytosolic phospholipase A2 (Gene Name=PLA2G4A)

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