Structure of PDB 1buc Chain A

Receptor sequence

>1bucA (length=383) Species: 907 (Megasphaera elsdenii)

MDFNLTDIQQDFLKLAHDFGEKKLAPTVTERDHKGIYDKELIDELLSLGI
TGAYFEEKYGGSGDDGGDVLSYILAVEELAKYDAGVAITLSATVSLCANP
IWQFGTEAQKEKFLVPLVEGTKLGAFGLTEPNAGTDASGQQTIATKNDDG
TYTLNGSKIFITNGGAADIYIVFAMTDKSKGNHGITAFILEDGTPGFTYG
KKEDKMGIHTSQTMELVFQDVKVPAENMLGEEGKGFKIAMMTLDGGRIGV
AAQALGIAEAALADAVEYSKQRVQFGKPLCKFQSISFKLADMKMQIEAAR
NLVYKAACKKQEGKPFTVDAAIAKRVASDVAMRVTTEAVQIFGGYGYSEE
YPVARHMRDAKITQIYEGTNEVQLMVTGGALLR

3D structure

• PDB: 1buc Three-dimensional structure of butyryl-CoA dehydrogenase from Megasphaera elsdenii.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L128 T129 G246 E367 G379
• Catalytic site (residue number reindexed from 1): L128 T129 G246 E367 G379
• Enzyme Commision number: 1.3.8.1: short-chain acyl-CoA dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CAA	A	L96 F126 G134 T135 T162 N182 H183 F236 M240 M241 L243 D244 T317 Y366 E367 G368	L96 F126 G134 T135 T162 N182 H183 F236 M240 M241 L243 D244 T317 Y366 E367 G368
BS02	FAD	A	F126 L128 T129 G134 T135 F160 T162 Y366 T369 E371 M375	F126 L128 T129 G134 T135 F160 T162 Y366 T369 E371 M375

Gene Ontology

Molecular Function

• GO:0003995 acyl-CoA dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0016937 short-chain fatty acyl-CoA dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0006631 fatty acid metabolic process

External links

• PDB: RCSB:1buc, PDBe:1buc, PDBj:1buc
• PDBsum: 1buc
• PubMed: 7857927
• UniProt: Q06319|ACDS_MEGEL Acyl-CoA dehydrogenase, short-chain specific