Structure of PDB 1bsu Chain A

Receptor sequence
>1bsuA (length=236) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKTYNINELNE
IPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDS
EDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGR
3D structure
PDB1bsu Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T37 N70 K92 T93 T94 Y95 T106 G109 T111 S112 K119 S183 T186 N188 T36 N69 K91 T92 T93 Y94 T105 G108 T110 S111 K118 S175 T178 N180 PDBbind-CN: Kd=230nM
BS02 dna A Q69 N70 S183 G184 N185 S223 Q68 N69 S175 G176 N177 S215 PDBbind-CN: Kd=230nM
BS03 CA A D74 D90 D73 D89
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1bsu, PDBe:1bsu, PDBj:1bsu
PDBsum1bsu
PubMed10074946
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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