Structure of PDB 1bss Chain A

Receptor sequence
>1bssA (length=230) Species: 562 (Escherichia coli) [Search protein sequence]
LRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPII
NKIAEKHGYIVEEPQQNHYPDFTLYKPSEPNKKIAIDIKATYTKIKFTLG
GYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKTYNINELNEIPKPYKG
VKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDY
WRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1bss Metal ion-mediated substrate-assisted catalysis in type II restriction endonucleases
ChainA
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A N70 K92 Y95 T106 G109 T111 S112 K119 N120 S183 T186 N188 N67 K89 Y92 T98 G101 T103 S104 K111 N112 S168 T171 N173
BS02 dna A Q69 L180 S183 G184 N185 S223 R226 N231 Q66 L165 S168 G169 N170 S208 R211 N216
BS03 CA A E45 D74 E43 D71
BS04 CA A D74 D90 I91 D71 D87 I88
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1bss, PDBe:1bss, PDBj:1bss
PDBsum1bss
PubMed9811827
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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