Structure of PDB 1bky Chain A

Receptor sequence
>1bkyA (length=291) Species: 10245 (Vaccinia virus) [Search protein sequence]
MDVVSLDKPFMYFEEIDNELDYEPESANEVAKKLPYQGQLKLLLGELFFL
SKLQRHGILDGATVVYIGSAPGTHIRYLRDHFYNLGVIIKWMLIDGRHHD
PILNGLRDVTLVTRFVDEEYLRSIKKQLHPSKIILISDVRSPSTADLLSN
YALQNVMISILNPVASSLKWRCPFPDQWIKDFYIPHGNKMLQPFAPSYSA
EMRLLSIYTGENMRLTRVTKSDAVNYEKKMYYLNKIVRNKVVVNFDYPNQ
EYDYFHMYFMLRTVYCNKTFPTTKAKVLFLQQSIFRFLNIP
3D structure
PDB1bky mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.57: methyltransferase cap1.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A Q39 L42 Y66 G68 S69 A70 P71 G72 H74 D95 R97 F115 V116 D138 V139 R140 Q39 L42 Y66 G68 S69 A70 P71 G72 H74 D95 R97 F115 V116 D138 V139 R140
BS02 1MC A Y22 F180 Y22 F174 MOAD: Kd=144uM
PDBbind-CN: -logKd/Ki=3.84,Kd=144uM
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003746 translation elongation factor activity
GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
GO:0008168 methyltransferase activity
Biological Process
GO:0006370 7-methylguanosine mRNA capping
GO:0006412 translation
GO:0006414 translational elongation
GO:0031440 regulation of mRNA 3'-end processing
GO:0032259 methylation
Cellular Component
GO:0044423 virion component

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1bky, PDBe:1bky, PDBj:1bky
PDBsum1bky
PubMed10377383
UniProtP07617|MCE_VACCW Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase (Gene Name=OPG102)

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