Structure of PDB 1bif Chain A

Receptor sequence
>1bifA (length=432) Species: 10116 (Rattus norvegicus) [Search protein sequence]
CPTLIVMVGLPARGKTYISKKLTRYLNFIGVPTREFNVGQYRRDMVKTYK
SFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRER
RAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEA
TEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSR
IVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQ
FISDQNIKDLKVFTSQMKRTIQTAEALSVPYEQFKVLNEIDAGVCEEMTY
EEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVL
VICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFL
NVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ
3D structure
PDB1bif The crystal structure of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase reveals distinct domain homologies.
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R255 H256 N262 R305 E325 H390
Catalytic site (residue number reindexed from 1) R219 H220 N226 R269 E289 H354
Enzyme Commision number 2.7.1.105: 6-phosphofructo-2-kinase.
3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PO4 A R255 H256 R305 E325 H390 R219 H220 R269 E289 H354
BS02 PO4 A Y336 R350 K354 Y365 R395 Y300 R314 K318 Y329 R359
BS03 AGS A P47 A48 R49 G50 K51 T52 Y53 N167 Q170 K172 V220 V246 Y427 P11 A12 R13 G14 K15 T16 Y17 N131 Q134 K136 V184 V210 Y391
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0003873 6-phosphofructo-2-kinase activity
GO:0004331 fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016787 hydrolase activity
Biological Process
GO:0006000 fructose metabolic process
GO:0006003 fructose 2,6-bisphosphate metabolic process
GO:0016310 phosphorylation
GO:0046835 carbohydrate phosphorylation
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1bif, PDBe:1bif, PDBj:1bif
PDBsum1bif
PubMed8805587
UniProtP25114|F264_RAT 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (Gene Name=Pfkfb4)

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