Structure of PDB 1bhm Chain A

Receptor sequence
>1bhmA (length=198) Species: 1390 (Bacillus amyloliquefaciens) [Search protein sequence]
MEVEKEFITDEAKELLSKDKLIQQAYNEVKTSICSPIWPATSKTFTINNT
EKNCNGVVPIKELCYTLLEDTYNWYREKPLDILKLEKKKGGPIDVYKEFI
ENSELKRVGMEFETGNISSAHRSMNKLLLGLKHGEIDLAIILMPIKQLAY
YLTDRVTNFEELEPYFELTEGQPFIFIGFNAEAYNSNVPLIPKGSDGM
3D structure
PDB1bhm Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding.
ChainA
Resolution2.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K146 Y150 R155 G197 K146 Y150 R155 G197
BS02 dna A V57 K61 K89 G90 G91 T114 N116 R122 K126 T153 D154 R155 K193 G194 D196 G197 M198 V57 K61 K89 G90 G91 T114 N116 R122 K126 T153 D154 R155 K193 G194 D196 G197 M198
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1bhm, PDBe:1bhm, PDBj:1bhm
PDBsum1bhm
PubMed7624794
UniProtP23940|T2BA_BACAM Type II restriction enzyme BamHI (Gene Name=bamHIR)

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