Structure of PDB 1bgb Chain A

Receptor sequence
>1bgbA (length=237) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKTYNINELNE
IPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDS
EDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1bgb Recognition of flanking DNA sequences by EcoRV endonuclease involves alternative patterns of water-mediated contacts.
ChainA
Resolution2.0 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A Q69 S183 G184 N185 R226 Q68 S175 G176 N177 R218
BS02 dna A T37 N70 K92 T93 T94 T106 T111 S112 K119 S183 T186 N188 T36 N69 K91 T92 T93 T105 T110 S111 K118 S175 T178 N180
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1bgb, PDBe:1bgb, PDBj:1bgb
PDBsum1bgb
PubMed9705308
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

[Back to BioLiP]