Structure of PDB 1bf2 Chain A

Receptor sequence

>1bf2A (length=750) Species: 32043 (Pseudomonas sp. 'amyloderamosa')

AINSMSLGASYDAQQANITFRVYSSQATRIVLYLYSAGYGVQESATYTLS
PAGSGVWAVTVPVSSIKAAGITGAVYYGYRAWGPNWPYASNWGKGSQAGF
VSDVDANGDRFNPNKLLLDPYAQEVSQDPLNPSNQNGNVFASGASYRTTD
SGIYAPKGVVLVPSTQSTGTKPTRAQKDDVIYEVHVRGFTEQDTSIPAQY
RGTYYGAGLKASYLASLGVTAVEFLPVQETQNDANDVVPNSDANQNYWGY
MTENYFSPDRRYAYNKAAGGPTAEFQAMVQAFHNAGIKVYMDVVYNHTAE
GGTWTSSDPTTATIYSWRGLDNATYYELTSGNQYFYDNTGIGANFNTYNT
VAQNLIVDSLAYWANTMGVDGFRFDLASVLGNSCLNGAYTASAPNCPNGG
YNFDAADSNVAINRILREFTVRPAAGGSGLDLFAEPWAIGGNSYQLGGFP
QGWSEWNGLFRDSLRQAQNELGSMTIYVTQDANDFSGSSNLFQSSGRSPW
NSINFIDVHDGMTLKDVYSCNGANNSQAWPYGPSDGGTSTNYSWDQGMSA
GTGAAVDQRRAARTGMAFEMLSAGTPLMQGGDEYLRTLQCNNNAYNLDSS
ANWLTYSWTTDQSNFYTFAQRLIAFRKAHPALRPSSWYSGSQLTWYQPSG
AVADSNYWNNTSNYAIAYAINGPSLGDSNSIYVAYNGWSSSVTFTLPAPP
SGTQWYRVTDTCDWNDGASTFVAPGSETLIGGAGTTYGQCGQSLLLLISK

3D structure

• PDB: 1bf2 Three-dimensional structure of Pseudomonas isoamylase at 2.2 A resolution.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D128 E229 T230 N232 D259 D375 E435 D510
• Catalytic site (residue number reindexed from 1): D128 E229 T230 N232 D259 D375 E435 D510
• Enzyme Commision number: 3.2.1.68: isoamylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D128 E229 T230 N232 D259	D128 E229 T230 N232 D259

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0019156 isoamylase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:1bf2, PDBe:1bf2, PDBj:1bf2
• PDBsum: 1bf2
• PubMed: 9719642
• UniProt: P10342|ISOA_PSEAY Isoamylase (Gene Name=iam)